Marovitz W, Loucopoulos A, Satyaswaroop P G, Gurpide E, Todd R, Naftolin F
Am J Obstet Gynecol. 1980 Nov 15;138(6):643-7. doi: 10.1016/0002-9378(80)90081-2.
Immunohistochemical techniques were used to search for the presence of 17beta-estradiol dehydrogenase activity in human endometrial and placental tissues, with the use of antibodies raised against highly purified human placental 17beta-estradiol dehydrogenase. Sensitivity and specificity of the antibodies were documented by radioimmunoassay and immunodiffusion on cellulose acetate. Although staining was consistently demonstrated in the syncytiotrophoblast layer of term placentas, in both cytoplasm and nuclei, no immunohistochemical reaction was observed in endometrial samples. These results support the contention that placental 17beta-estradiol dehydrogenase is immunologically dissimilar from the endometrial enzyme.
利用针对高度纯化的人胎盘17β-雌二醇脱氢酶产生的抗体，采用免疫组织化学技术检测人子宫内膜和胎盘组织中17β-雌二醇脱氢酶活性的存在情况。通过放射免疫分析和醋酸纤维素上的免疫扩散记录抗体的敏感性和特异性。尽管足月胎盘的合体滋养层在细胞质和细胞核中均持续出现染色，但子宫内膜样本中未观察到免疫组织化学反应。这些结果支持了胎盘17β-雌二醇脱氢酶在免疫学上与子宫内膜酶不同的观点。
