体外基底层形成可能需要非表皮细胞的生存底物。

In vitro basal lamina formation may require non-epidermal cell living substrate.

作者信息

Woodley D, Régnier M, Pruniéras M

出版信息

Br J Dermatol. 1980 Oct;103(4):397-404. doi: 10.1111/j.1365-2133.1980.tb07262.x.

DOI:10.1111/j.1365-2133.1980.tb07262.x

Abstract

In an adult human epidermal cell culture system in which three different types of dermo-epidermal junctions could be observed, the in vitro synthesis of basement membrane and type IV collagen was studied with electron microscopy and indirect immunofluorescence via an antiserum to type IV collagen. Essentially, keratinocytes juxtaposed to non-living substrate did not produce either type IV collagen or an ultrastructural basement membrane, whereas both products were found at dermo-epidermal junctions composed of living keratinocytes juxtaposed to living dermis. This suggests that the microenvironment of the keratinocyte may influence the synthesis of junctional components.

摘要

在一个能观察到三种不同类型真皮 - 表皮连接的成人人类表皮细胞培养系统中，通过抗IV型胶原抗血清，利用电子显微镜和间接免疫荧光研究了基底膜和IV型胶原的体外合成。从本质上讲，与无生命底物并列的角质形成细胞既不产生IV型胶原，也不产生超微结构的基底膜，但在由与活真皮并列的活角质形成细胞组成的真皮 - 表皮连接处发现了这两种产物。这表明角质形成细胞的微环境可能影响连接成分的合成。