Chamson A, Germain N, Claudy A, Perier C, Frey J
Biochemistry Department, Medical School, Saint-Etienne, France.
Arch Dermatol Res. 1989;281(4):267-72. doi: 10.1007/BF00431061.
Two different dermal-epidermal recombinants were prepared in vitro and used to study the synthesis and formation of basement membrane. The first was obtained by culturing keratinocytes on the surface of a collagen lattice populated by fibroblasts. The second was prepared by coculture of both keratinocytes and fibroblasts in a collagen lattice. After 6 weeks of culture, the basal lamina was observed with electron microscopy only if keratinocytes were cultivated on top of the collagen lattice populated by fibroblasts. In the second model, however, type IV collagen, laminin, and pemphigoid bullosa antigen could be detected by immunofluorescence as well as synthesis of type IV collagen in the culture, but no basement membrane was observed by electron microscopy. These data demonstrate that, in vitro, basement membrane formation depends not only on the presence of the macromolecular components but also on the culture conditions.
在体外制备了两种不同的真皮 - 表皮重组体，并用于研究基底膜的合成与形成。第一种是通过在由成纤维细胞构成的胶原晶格表面培养角质形成细胞获得的。第二种是通过在胶原晶格中将角质形成细胞和成纤维细胞共同培养制备的。培养6周后，仅当角质形成细胞培养在由成纤维细胞构成的胶原晶格顶部时，才能通过电子显微镜观察到基底层。然而，在第二种模型中，通过免疫荧光可检测到IV型胶原、层粘连蛋白和类天疱疮抗原，并且在培养物中可检测到IV型胶原的合成，但通过电子显微镜未观察到基底膜。这些数据表明，在体外，基底膜的形成不仅取决于大分子成分的存在，还取决于培养条件。
