Hill M W, Mackenzie I C
Cell Tissue Res. 1984;237(3):473-8. doi: 10.1007/BF00228431.
The interaction between adult stratified squamous epithelium and its supporting connective tissue possibly involves both permissive and directive influences. To examine the effect of vitality and specificity of connective tissue on the maintenance of epithelial structure and histodifferentiation, specimens of skin and oral mucosa from various regions of adult mice were separated using either EDTA or trypsin. Prior to transplantation, the epithelium was recombined with either inverted homologous connective tissue or with connective tissue that had been killed either by heating or repeated freeze-thawing. Epithelial sheets were also transplanted onto the graft bed alone or in combination with striated muscle or tendon. Normal patterns of cytodifferentiation were maintained when the epithelium was recombined with inverted or frozen-thawed subepithelial connective tissue but there was a loss of spatial organization on the frozen-thawed connective tissue. In contrast, heat-killed or trypsin-treated frozen-thawed subepithelial connective tissue and non-dermal connective tissue failed to maintain a viable epithelium. These observations suggest that subepithelial connective tissues (dermis, lamina propria) but not deep connective tissues facilitate epithelial proliferation and histodifferentiation.
成人复层鳞状上皮与其支持性结缔组织之间的相互作用可能涉及许可性和指导性影响。为了研究结缔组织的活力和特异性对上皮结构维持和组织分化的影响，使用乙二胺四乙酸（EDTA）或胰蛋白酶分离成年小鼠不同部位的皮肤和口腔黏膜标本。在移植前，将上皮与倒置的同源结缔组织或经加热或反复冻融杀死的结缔组织重新组合。上皮片也单独移植到移植床或与横纹肌或肌腱联合移植。当上皮与倒置或冻融的上皮下结缔组织重新组合时，细胞分化的正常模式得以维持，但在冻融的结缔组织上空间组织丧失。相比之下，热灭活或胰蛋白酶处理的冻融上皮下结缔组织和非真皮结缔组织无法维持存活的上皮。这些观察结果表明，上皮下结缔组织（真皮、固有层）而非深层结缔组织促进上皮增殖和组织分化。
