Kobayashi M, Ohgaku S, Iwasaki M, Harano Y, Maegawa H, Shigeta Y
Endocrinol Jpn. 1980 Jun;27(3):337-42. doi: 10.1507/endocrj1954.27.337.
A modified erythrocyte insulin receptor assay was developed. The major advantage of our method is the smaller amount of blood necessary for the assay and consequent simple handling in the binding assay. Three ml of blood is enough for this assay, making it possible to assess insulin receptors in a group of pediatric patients. There was a close relationship between mononuclear cells and erythrocyte insulin binding assays (r = 0.900, p < 0.05). With this method insulin binding studies were done on normal subjects and adult onset non-obese diabetics. Decreased insulin binding was seen in the diabetic group, and this was due to a decreased number of insulin receptors. This erythrocyte insulin receptor assay can be useful tool for the study of the mechanism of insulin resistance.
开发了一种改良的红细胞胰岛素受体测定法。我们方法的主要优点是测定所需的血量较少，因此在结合测定中操作简单。3毫升血液就足以进行此测定，从而能够对一组儿科患者的胰岛素受体进行评估。单核细胞与红细胞胰岛素结合测定之间存在密切关系（r = 0.900，p < 0.05）。用这种方法对正常受试者和成年起病的非肥胖糖尿病患者进行了胰岛素结合研究。糖尿病组中观察到胰岛素结合减少，这是由于胰岛素受体数量减少所致。这种红细胞胰岛素受体测定法可能是研究胰岛素抵抗机制的有用工具。
