Hrubá A, Paluska E, Fricová V, Fiala J
Folia Biol (Praha). 1980;26(5):338-50.
Anti-T antibody was obtained from xenogeneic antithymocyte serum by adsorption and elution with brain immunosorbent. Bone marrow and spleen cells from the parental A strain were treated in vitro with anti-T antibody and complement or with the original ATS and complement. So treated cells were assayed for the ability to induce a local and a total GVH reaction in (A X C57BL)F1 hybrid mice, to prolong the survival of lethally irradiated, identical F1 hybrid mice and to form haemopoietic colonies in spleens of the syngeneic irradiated recipients. The local GVH reaction induced by anti-T antibody- and ATS-treated spleen cells was somewhat lower. Administration of a mixture of spleen and bone marrow cells treated with anti-T antibody prolonged the survival of lethally irradiated recipients but did not prevent the consequences of late GVH reaction. The colony-forming activity of bone marrow cells treated with anti-T antibody was not reduced in most experiments. It can be concluded that the treatment of cells from mouse haemopoietic organs with anti-T antibody and complement damages mature T lymphocytes and in this way reduces the ability to induce GVH reaction, but does not damage T lymphocyte precursors and haemopoietic stem cells.
通过用脑免疫吸附剂吸附和洗脱，从异种抗胸腺细胞血清中获得抗 - T 抗体。将亲代 A 品系的骨髓和脾细胞在体外分别用抗 - T 抗体和补体或原始抗胸腺细胞血清和补体处理。对如此处理过的细胞进行检测，以评估其在（A×C57BL）F1 杂种小鼠中诱导局部和全身移植物抗宿主反应（GVH）的能力、延长经致死剂量照射的同基因 F1 杂种小鼠存活时间的能力以及在同基因受照射受体脾脏中形成造血集落的能力。用抗 - T 抗体和抗胸腺细胞血清处理的脾细胞诱导的局部 GVH 反应略低。给予用抗 - T 抗体处理的脾细胞和骨髓细胞混合物可延长经致死剂量照射受体的存活时间，但不能预防晚期 GVH 反应的后果。在大多数实验中，用抗 - T 抗体处理的骨髓细胞的集落形成活性并未降低。可以得出结论，用抗 - T 抗体和补体处理小鼠造血器官的细胞会损伤成熟的 T 淋巴细胞，从而降低诱导 GVH 反应的能力，但不会损伤 T 淋巴细胞前体和造血干细胞。
