Cox B S, Tuite M F, Mundy C J
Genetics. 1980 Jul;95(3):589-609. doi: 10.1093/genetics/95.3.589.
Reversion from the suppressed to nonsuppressed phenotype in strains of geno;type SUQ5 [psi+] ade2-1 his5-2 lys1-1 can1-100 ura3-1 has been induced by treatment with ethyl methanesulphonate, nitrosoguanidine or UV (254 nm) light. Spontaneously occurring revertants have also been selected by two different methods. Reversion has been shown to occur through a variety of nuclear mutations and through mutation of [psi+] to [psi-]. Nuclear mutations included back-mutation of SUQ5, antisuppressor mutations that were recessive, semi-dominant or dominant, and dominant or recessive mutations of genes required for the maintenance of the [psi+] factor. Complementation tests by which the various kinds of mutations could be distinguished from one another were designed. The spectra of spontaneously occurring and induced mutations have been described.
基因型为SUQ5 [psi+] ade2-1 his5-2 lys1-1 can1-100 ura3-1的菌株从抑制表型回复到非抑制表型，可通过甲磺酸乙酯、亚硝基胍处理或紫外线（254纳米）照射诱导产生。自发出现的回复突变体也通过两种不同方法筛选得到。回复突变已被证明可通过多种核突变以及[psi+]突变为[psi-]发生。核突变包括SUQ5的回复突变、隐性、半显性或显性的反抑制突变，以及维持[psi+]因子所需基因的显性或隐性突变。设计了互补试验以区分各种不同类型的突变。已描述了自发出现的突变和诱导突变的谱型。
