Jones J M
Infect Immun. 1980 Oct;30(1):78-89. doi: 10.1128/iai.30.1.78-89.1980.
Cell wall mannan of type A Candida albicans was purified, conjugated with tyramine, and labeled with 125I. Labeled cell wall mannan was used in a radioimmunoassay to measure serum antimannan antibody levels. An ammonium sulfate-soluble fraction of a cytoplasmic extract of C. albicans contained a large amount of a major cytoplasmic antigen of this organism. When the sulfate-soluble fraction was labeled with 125I, much more 125I attached to this major antigen than to the other antigens present in the sulfate-soluble fraction. Thus, when serum antisulfate-soluble fraction antibody levels were measured by a radioimmunoassay which used the iodine-labeled sulfate-soluble fraction, antibody against this major cytoplasmic antigen was quantitated. Both radioimmunoassays were used to measure antimannan and antisulfate-soluble fraction antibody levels in mice, rabbits, and humans. Irrespective of the procedure used to elicit antibody against C. albicans antigens, mice failed to produce antimannan antibody. By contrast, all strains of mice tested produced antisulfate-soluble fraction antibody after immunization, and the magnitude of this antibody response depended on the strain of mice immunized. Rabbits readily produced antibody against both mannan and sulfate-soluble fraction when immunized by a variety of methods. Antimannan antibody was detected in 100% of sera from a randomly selected sample of 50 hospitalized patients. Only 1 of 50 patients had antisulfate-soluble fraction antibody detectable by radioimmunoassay. In pooled normal human serum, most antimannan antibody was of the immunoglobulin G class.
白色念珠菌A型细胞壁甘露聚糖被纯化，与酪胺偶联，并进行¹²⁵I标记。标记后的细胞壁甘露聚糖用于放射免疫测定，以检测血清抗甘露聚糖抗体水平。白色念珠菌细胞质提取物的硫酸铵可溶部分含有大量该菌的一种主要细胞质抗原。当该硫酸可溶部分用¹²⁵I标记时，附着在这种主要抗原上的¹²⁵I比附着在硫酸可溶部分中其他抗原上的¹²⁵I多得多。因此，当用碘标记的硫酸可溶部分通过放射免疫测定来检测血清抗硫酸可溶部分抗体水平时，就能对针对这种主要细胞质抗原的抗体进行定量。这两种放射免疫测定法都用于检测小鼠、兔子和人类血清中的抗甘露聚糖抗体及抗硫酸可溶部分抗体水平。无论采用何种方法诱导针对白色念珠菌抗原的抗体产生，小鼠都不能产生抗甘露聚糖抗体。相比之下，所有测试的小鼠品系在免疫后都产生了抗硫酸可溶部分抗体，且这种抗体反应的强度取决于所免疫的小鼠品系。兔子通过多种方法免疫后很容易产生针对甘露聚糖和硫酸可溶部分的抗体。在随机抽取的50名住院患者的血清样本中，100%检测到抗甘露聚糖抗体。50名患者中只有1人通过放射免疫测定法检测到抗硫酸可溶部分抗体。在正常人混合血清中，大多数抗甘露聚糖抗体属于免疫球蛋白G类。
