Cahen D, Bults G, Garty H, Malkin S
J Biochem Biophys Methods. 1980 Nov;3(5):293-310. doi: 10.1016/0165-022x(80)90010-x.
Photoacoustic (PA) measurements provide, by the very nature of the PA effect, the possibility to obtain information on the optical and thermal properties of samples. In addition they can yield information on the enthalpy changes and characteristic times involved in photo-induced processes as the acoustic signal is proportional to the hear produced following the absorption of the modulated excitation. In the study of optical properties the relative insensitivity to scattered light of the PA signal makes such measurement an attractive way to measure biological samples in vivo, or, at least, without the need to isolate the absorbing compounds. The dependence of the PA signal on the thermal properties of the sample is particularly useful when heterogeneous samples are studied. As a photocalorimetric method the technique shows considerable promise in the study of photo-bioenergetics, especially photosynthesis. Only in special cases can analytical applications of the PA method compete with fluorescence measurements for detection, and with increasingly sophisticated optical transmission and reflectance techniques (for identification).. However, the PA method may find important uses in fundamental research and in applied areas such as biomedicine and agricultural biochemistry.
光声（PA）测量基于光声效应的本质，提供了获取样品光学和热学性质信息的可能性。此外，由于声信号与调制激发吸收后产生的热量成正比，它们还能提供有关光诱导过程中焓变和特征时间的信息。在光学性质研究中，光声信号对散射光相对不敏感，使得这种测量成为体内测量生物样品的一种有吸引力的方法，或者至少无需分离吸收化合物。当研究异质样品时，光声信号对样品热学性质的依赖性特别有用。作为一种光量热法，该技术在光生物能量学，特别是光合作用的研究中显示出巨大的前景。只有在特殊情况下，光声方法的分析应用才能在检测方面与荧光测量竞争，以及在识别方面与日益复杂的光透射和反射技术竞争。然而，光声方法可能在基础研究以及生物医学和农业生物化学等应用领域找到重要用途。
