巨噬细胞细胞表面放射性碘化的改进
Improved cell surface radioiodination of macrophages.
作者信息
Kulczycki A, Krause V, Killion C C, Atkinson J P
出版信息
J Immunol Methods. 1980;37(2):133-8. doi: 10.1016/0022-1759(80)90198-2.
Rabbit alveolar macrophages at high cell concentrations are inefficiently radiolabeled by the usual lactoperoxidase-catalyzed iodination method. Soluble macromolecules secreted by macrophages were found to inhibit the radioiodination of macrophages and other cells. A modified procedure is described which minimizes the presence of such inhibitory material and thereby considerably improves the radioiodination efficiency for both alveolar macrophages and the macrophage-like P388D1 cell line.
在高细胞浓度下，兔肺泡巨噬细胞用常规的乳过氧化物酶催化碘化法进行放射性标记的效率较低。研究发现，巨噬细胞分泌的可溶性大分子会抑制巨噬细胞和其他细胞的放射性碘化。本文描述了一种改进方法，该方法可尽量减少此类抑制性物质的存在，从而显著提高肺泡巨噬细胞和巨噬细胞样P388D1细胞系的放射性碘化效率。
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