Mattiasson B, Ling T G
J Immunol Methods. 1980;38(3-4):217-23. doi: 10.1016/0022-1759(80)90269-0.
A two-phase partitioning technique was used to separate free from bound labelled ligand in a binding assay. Efficient separation was accomplished by modifying one of the reactants in such a way that it partitions preferentially to one of the two phases. The biospecific interactions between the lectin concanavalin A and various carbohydrates and glycoproteins were investigated in this model study.
在一项结合测定中，采用两相分配技术来分离游离的和结合的标记配体。通过对其中一种反应物进行修饰，使其优先分配到两相中的一相，从而实现了高效分离。在该模型研究中，对凝集素伴刀豆球蛋白A与各种碳水化合物和糖蛋白之间的生物特异性相互作用进行了研究。
