Pure and Applied Biochemistry, Chemical Center, University of Lund, PO Box 740, S-22007, Lund, Sweden.
Appl Biochem Biotechnol. 1982 Jan;7(1-2):71-4. doi: 10.1007/BF02798626.
In a competitive binding assay, the ligand to be quantified competes with a fixed amount of labeled ligand for the sites on a limiting amount of binding protein. The amount of label bound is therefore dependent on the ratio between native and labeled ligand. In a binding assay, one must separate the free ligands from bound. The better the separation, the higher the sensitivity of the assay. But effective methods are often laborious and time-consuming and thus we have developed a novel approach, the Partition Affinity Ligand Assay (PALA).
在竞争结合分析中，待定量的配体与固定量的标记配体竞争结合有限量的结合蛋白上的结合位点。因此，标记结合的量取决于天然配体和标记配体之间的比例。在结合分析中，必须将游离配体与结合配体分离。分离效果越好，分析的灵敏度越高。但是，有效的方法往往繁琐且耗时，因此我们开发了一种新方法，即分配亲和力配体分析（PALA）。
