使用磷酸胆碱衍生化的绵羊红细胞在体外诱导克隆限制的胸腺依赖性抗体反应。

Induction of clonally restricted thymus-dependent antibody responses in vitro using phosphorylcholine derivatized sheep erythrocytes.

作者信息

Cambier J C, Neale M J

出版信息

J Immunol Methods. 1980;38(3-4):231-8. doi: 10.1016/0022-1759(80)90271-9.

Abstract

Induction of anti-phosphorylcholine responses in vitro using phosphorylcholine derivatized sheep erythrocytes (SRBC) is described. These responses are thymus dependent and restricted to expression of the TEPC 15 idiotype. Optimal B-cell concentrations in cultures were shown to be different for elicitation of direct anti-PC PFC responses than for elicitation of PFC specific for carrier (SRBC) determinants. Similarly, these responses differed in optimal immunogen concentration requirements. These differences presumably relate to the clonality of the respective responses. The methodology described provides an alternative to induction of clonally restricted thymus-dependent responses in vitro using PC derivatized soluble protein antigens. Induction of responses using PC-SRBC has the advantages of the relative case of T-cell priming to SRBC and concurrent stimulation of monoclonal anti-PC responses and easily assayed polyclonal anti-SRBC responses.

摘要

本文描述了使用磷酸胆碱衍生化的绵羊红细胞（SRBC）在体外诱导抗磷酸胆碱反应的方法。这些反应依赖胸腺，并局限于TEPC 15独特型的表达。结果显示，培养物中引发直接抗PC PFC反应的最佳B细胞浓度与引发针对载体（SRBC）决定簇的PFC的最佳B细胞浓度不同。同样，这些反应在最佳免疫原浓度要求上也有所不同。这些差异可能与各自反应的克隆性有关。所描述的方法为使用PC衍生化的可溶性蛋白抗原在体外诱导克隆受限的胸腺依赖性反应提供了一种替代方法。使用PC-SRBC诱导反应具有以下优点：相对容易对SRBC进行T细胞致敏，同时刺激单克隆抗PC反应以及易于检测的多克隆抗SRBC反应。