Improved method for detection of anti-tissue antibody by indirect immunofluorescence using fixed tissue.

Techniques have been developed which allow paraffin embedded, fixed tissue to be used for indirect immunofluorescence. Antibody to parietal cells, mitochondria, actin, adrenal gland, parathyroid tissue, thyroglobulin, thyroid microsomes, and pancreas islet cells cen be detected. The procedure offers improved histology and decreased technical time for the indirect immunofluorescence technique.