用于合成对应于大肠杆菌甲硫氨酸起始tRNA第21 - 34位碱基的十四聚核苷酸的RNA连接酶反应的底物碱基序列比较1。
Comparison of substrate base sequences for RNA ligase reactions in the synthesis of a tetradecanucleotide corresponding to bases 21-34 of E. coli tRNAfMet 1.
作者信息
Ohtsuka E, Doi T, Uemura H, Taniyama Y, Ikehara M
出版信息
Nucleic Acids Res. 1980 Sep 11;8(17):3909-16. doi: 10.1093/nar/8.17.3909.
A tetradecanucleotide U-A-G-C(U-C-G)2G-G-C-Up corresponding to bases 21-34 of a nascent sequence of formylmethionyl tRNA of E. coli has been synthesized by the joining of two combinations of chemically synthesized oligonucleotides: 1) U-A-G-C + U-C-G-U-C-G + G-G-C-Up and 2) U-A-G-C + U-C-G-U + C-G-G-G-C-Up. In reaction 1) and the extent of joining *pG-G-C-Up to U-C-G-U-C-G was only 15.4% and the last ligation of the decamer to U-A-G-U proceeded to 27%. In reaction 2) joining between U-A-G-C and pU-C-G-Up gave a high yield (88%). The ligation of this octamer and *pC-G-G-G-C-Up also gave a satisfactory yield (52%). These reactions suggest that sequence preferences in RNA ligase reactions may arise from the structure of the 3'-end of acceptor molecules.
一个对应于大肠杆菌甲酰甲硫氨酰tRNA新生序列第21至34位碱基的十四聚体U-A-G-C(U-C-G)2G-G-C-Up，是通过连接化学合成的寡核苷酸的两种组合而合成的：1) U-A-G-C + U-C-G-U-C-G + G-G-C-Up和2) U-A-G-C + U-C-G-U + C-G-G-G-C-Up。在反应1)中，pG-G-C-Up与U-C-G-U-C-G的连接程度仅为15.4%，而十聚体与U-A-G-U的最后连接达到27%。在反应2)中，U-A-G-C与pU-C-G-Up之间的连接产率很高(88%)。该八聚体与pC-G-G-G-C-Up的连接产率也令人满意(52%)。这些反应表明，RNA连接酶反应中的序列偏好可能源于受体分子3'末端的结构。
Zotero 插件