[高纯度F型肉毒梭菌毒素的分离及特性]

[Isolation and properties of a highly purified Clostridium botulinum type F toxin].

作者信息

Uvarova R N, Reshetnikova L N, Ispolatovskaia M V, Bulatova T I

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1980 Nov(11):42-6.

Abstract

A scheme for isolation of highly purified type F Cl. botulinum toxin has been developed. The scheme allows one to obtain the toxin with specific activity of 1.5--4.0 X 10(7) Dlm/mg protein, molecular weight of 150,000 daltons, a typical protein UV spectrum and the optical density ratio E280/E260 = 1.98.

摘要

已开发出一种用于分离高纯度F型肉毒杆菌毒素的方案。该方案能够获得比活性为1.5--4.0×10(7) Dlm/mg蛋白质、分子量为150,000道尔顿、具有典型蛋白质紫外光谱且光密度比E280/E260 = 1.98的毒素。