抗F型肉毒梭菌毒素单克隆抗体。
Monoclonal antibody to type F Clostridium botulinum toxin.
作者信息
Ferreira J L, Hamdy M K, McCay S G, Zapatka F A
机构信息
Food and Drug Administration, Atlanta, Georgia 30309.
出版信息
Appl Environ Microbiol. 1990 Mar;56(3):808-11. doi: 10.1128/aem.56.3.808-811.1990.
Abstract
Hybridomas synthesizing monoclonal antibodies (MAbs) against type F Clostridium botulinum toxin were developed. MAb from one stable hybridoma, hybridoma 223, consisted of kappa light chains and an immunoglobulin G subclass 2a heavy chain. This MAb was used in a double-sandwich enzyme-linked immunosorbent assay to detect type F toxin in foods, culture fluids, and purified toxin preparations. The sensitivity of the double-sandwich enzyme-linked immunosorbent assay was approximately 10 mouse lethal doses of toxin per ml of toxic fluid.
摘要
已开发出能合成抗F型肉毒梭菌毒素单克隆抗体（MAb）的杂交瘤。来自一个稳定杂交瘤（杂交瘤223）的单克隆抗体由κ轻链和免疫球蛋白G 2a亚类重链组成。该单克隆抗体用于双夹心酶联免疫吸附测定，以检测食品、培养液和纯化毒素制剂中的F型毒素。双夹心酶联免疫吸附测定的灵敏度约为每毫升有毒液体10个小鼠致死剂量的毒素。
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