Eshmantaĭte N A, Rimkunas A I, Shaulauskene I I, Azhukaĭtene R P
Zh Mikrobiol Epidemiol Immunobiol. 1980 Nov(11):80-3.
The interaction between 2 strains of group A streptococci in L-forms and the cells of the continuous cell lines HEp, HeLa, L, GPK and PEK, as well as the cells of primary human and chick embryo cell cultures was studied under conditions of infection with different doses. In most of the cell cultures used in this study L-form of streptococci showed no pronounced cytopathic effect. They could be isolated, when using cell cultures as inoculum, from the cultivated cells of continuous cell lines during the whole period of the cultivation of the infected monolayer (6--7 days), primary human embryo cell culture up to days 8--11 and from chick embryo cell culture up to days 1--3. In the cells of the continuous cell lines the maximum amount of L-forms was revealed on days 2--3 by the immunofluorescent technique. The ultrathin sections of HEp and HeLa cells infected with L-forms of streptococci were found to contain small elements similar to L-forms inside the cells and on their surface, which was not detected in the infected primary cell cultures.
研究了两株A组链球菌L型与连续细胞系HEp、HeLa、L、GPK和PEK的细胞以及原代人细胞和鸡胚细胞培养物在不同剂量感染条件下的相互作用。在本研究中使用的大多数细胞培养物中，链球菌L型未表现出明显的细胞病变效应。当以细胞培养物作为接种物时，在感染单层培养的整个期间（6 - 7天），可从连续细胞系的培养细胞中分离出链球菌L型；原代人胚细胞培养物在第8 - 11天可分离出；鸡胚细胞培养物在第1 - 3天可分离出。通过免疫荧光技术在连续细胞系的细胞中于第2 - 3天发现了最大量的L型。发现感染链球菌L型的HEp和HeLa细胞的超薄切片在细胞内部和表面含有类似于L型的小元件，而在感染的原代细胞培养物中未检测到。
