Ferrua B, Chavance C, Maiolini R, Krebs B P, Masseyeff R
Ann Biol Clin (Paris). 1980;38(4):237-42.
The authors present a kit marketed by Abbott for searching HBs antigen by an immunological method. This technic of sandwich type included 3 stages. During a first stage, the HBs antigen eventually present was extracted from the biological fluid by a specific antibody adsorbed on a polystyrène bead. After washing, a second specific antibody labelled with peroxidase was added. After incubation and washing, the peroxidase activity of the bead was revealed by adding a chromogenic substrate. The development of a colour indicated the presence of HBs antigen in the biological fluid under study. Very simple to use, this kit permits specific detection of HBs antigen with a sensitivity comparable to that of radio-immunoassay. A good correlation was obtained with the radio-immunological kit Austria II and with the enzymo-immunological kit Hepanostika.
作者介绍了雅培公司销售的一种用于通过免疫方法检测乙肝表面抗原的试剂盒。这种夹心型技术包括三个阶段。在第一阶段，最终存在的乙肝表面抗原通过吸附在聚苯乙烯珠上的特异性抗体从生物体液中提取出来。洗涤后，加入用辣根过氧化物酶标记的第二种特异性抗体。孵育和洗涤后，通过加入显色底物来显示珠子的过氧化物酶活性。颜色的出现表明所研究的生物体液中存在乙肝表面抗原。该试剂盒使用非常简单，能够特异性检测乙肝表面抗原，其灵敏度与放射免疫分析相当。与奥地利II型放射免疫试剂盒和酶免疫试剂盒Hepanostika有良好的相关性。
