Turner K J, Stewart G A, Sharp A H, Czarny D
Clin Allergy. 1980 Jul;10(4):441-50. doi: 10.1111/j.1365-2222.1980.tb02127.x.
Five allergen extracts of Dermatophagoides pteronyssinus, Lolium perenne, Alternaria tenuis, Aspergillus fumigatus and Cladosporium herbarum, obtained from four different manufacturers, were examined by inhibition of RAST, content of protein and carbohydrate, contents of phosphorylcholine (Pc) and tridacnin reactive components, and by skin test. Inhibition of RAST was used as a primary method for establishing allergenic potency and demonstrated wide variations for each preparation supplied by the different manufacturers. The extracts also varied widely in protein and carbohydrate content and in the ratio of these parameters, indicating internal heterogeneity. Pc content was significantly related to RAST potency for extracts of A. fumigatus and A. tenuis, suggesting that Pc content may be used as a primary standarization procedure for these extracts. Skin test reactions undertaken at a single concentration did not show any significant variation in weal size between preparations of a given allergen extract. However, of particular importance to practising clinicians is the finding that varying numbers of patients showed negative skin reactions to one preparation of a particular allergen yet were positive to the corresponding preparations supplied by the other companies.
对从四个不同厂家获得的五种变应原提取物（屋尘螨、黑麦草、细交链孢菌、烟曲霉和草本枝孢菌）进行了放射性变应原吸附试验（RAST）抑制、蛋白质和碳水化合物含量、磷酸胆碱（Pc）和三角蛤素反应性成分含量检测以及皮肤试验。RAST抑制试验被用作确定变应原效力的主要方法，结果显示不同厂家提供的每种制剂之间存在很大差异。提取物的蛋白质和碳水化合物含量以及这些参数的比例也有很大差异，表明其内部存在异质性。对于烟曲霉和细交链孢菌的提取物，Pc含量与RAST效力显著相关，这表明Pc含量可作为这些提取物的主要标准化程序。在单一浓度下进行的皮肤试验反应显示，给定变应原提取物的制剂之间风团大小没有任何显著差异。然而，对于临床医生来说特别重要的是，发现不同数量的患者对某一特定变应原的一种制剂皮肤反应呈阴性，但对其他公司提供的相应制剂呈阳性。
