Muronets E M, Kovtunenko L V, Kameneva S V
Genetika. 1980;16(7):1168-75.
Combined mutagenic effect of near ultraviolet irradiation (NUV, lambda > 320 nm) and angelicin, which forms monoadducts, or 8-methoxypsoralen (8-MOP), which forms monoadducts and cross-links in DNA, were studied in uvs and uvs+ strains of Aspergillus nidulans. Mutations were induced intensively by both kinds of furocumarins. The induction of mutations by angelicin testifies the significance of psoralen-pyrimidine monoadducts in mutagenesis. Using the method of fractionating NUV-irradiation and studying the influence of monoadducts repair to the mutation frequency, it is found the interstrand crosslinks of DNA induced by 8-MOP plus NUV are also nighly mutagenic. The products of genes uvs 12, uvs 20b and uvs 26, acting in DNA excision repair, do not participate in forming the mutations induced by furocumarins plus NUV in Aspergillus nidulans. The products of genes uvs 19, uvs 20a, participating in DNA post-replicative repair, are important for repair of premutational damages induced by furocumarins plus NUV.
在构巢曲霉的uvs和uvs +菌株中，研究了近紫外线照射（NUV，λ> 320 nm）与形成单加合物的当归素或在DNA中形成单加合物和交联的8-甲氧基补骨脂素（8-MOP）的联合诱变作用。两种呋喃香豆素均强烈诱导突变。当归素诱导突变证明了补骨脂素-嘧啶单加合物在诱变中的重要性。利用分馏NUV辐射的方法并研究单加合物修复对突变频率的影响，发现8-MOP加NUV诱导的DNA链间交联也具有高度诱变性。参与DNA切除修复的uvs 12、uvs 20b和uvs 26基因的产物不参与构巢曲霉中呋喃香豆素加NUV诱导的突变形成。参与DNA复制后修复的uvs 19、uvs 20a基因的产物对于修复呋喃香豆素加NUV诱导的突变前损伤很重要。
