Smith G R, Hooker J M, Milligan R A
J Hyg (Lond). 1980 Oct;85(2):247-56. doi: 10.1017/s0022172400063282.
Nine caprine and ovine mycoplasma strains, said to be indistinguishable serologically from Mycoplasma mycoides subsp. mycoides (the causative organism of contagious bovine pleuropneumonia; CBPP) were examined in mice by (1) a mycoplasmaemia test, and (2) a cross-protection test. Of the nine strains, two from goats belonged to a small colony (SC) type; four caprine and three ovine strains belonged to a large colony (LC) type.The two SC strains - like a single SC strain examined in an earlier study - were indistinguishable from genuine M. mycoides subsp. mycoides as isolated from CBPP. They produced mycoplasmaemia readily. In a cross-protection test, the two SC strains and a CBPP strain immunized completely against each other.Of the seven LC strains, six - like six LC strains examined in an earlier study - were easily distinguished from genuine M. mycoides subsp. mycoides; except for one that was not tested, all were shown to lack the ability to produce mycoplasmaemia readily. In cross-protection tests all six strains immunized partially but not completely against a CBPP strain.The seventh LC strain (Mankefår 2833) was exceptional: it produced mycoplasmaemia readily, resembling the SC strains in this respect. Like other LC strains, in cross-protection tests it protected only partially against a CBPP strain. Strain Mankefår 2833 was isolated in ca. 1965 by Brack from a Barbary sheep (Ammotragus lervia) in a German zoo.The ability of Mankefår 2833 to produce mycoplasmaemia enabled it to be used as a challenge strain in cross-protection tests. For the purpose of such tests the collection of nine mycoplasma strains referred to above was augmented with six LC strains from an earlier study. Partial but not complete protection against Mankefår 2833 was produced by two caprine SC strains, one CBPP strain, and nine LC strains. Three further LC strains gave protection that may have been as strong as that produced by the homologous strain, but confirmatory experiments are needed. A strain of M. mycoides subsp. capri gave no protection against Mankefår 2833.
对九株山羊和绵羊支原体菌株进行了研究，据说这些菌株在血清学上与丝状支原体丝状亚种（传染性牛胸膜肺炎的病原体；CBPP）无法区分。通过（1）支原体血症试验和（2）交叉保护试验在小鼠中对它们进行了检测。在这九株菌株中，两株来自山羊的属于小菌落（SC）型；四株山羊菌株和三株绵羊菌株属于大菌落（LC）型。这两株SC菌株——就像在早期研究中检测的一株单一SC菌株一样——与从CBPP分离出的真正丝状支原体丝状亚种无法区分。它们很容易产生支原体血症。在交叉保护试验中，这两株SC菌株和一株CBPP菌株相互提供了完全的保护。在七株LC菌株中，六株——就像在早期研究中检测的六株LC菌株一样——很容易与真正的丝状支原体丝状亚种区分开来；除了一株未进行检测外，所有菌株都显示出不容易产生支原体血症的能力。在交叉保护试验中，所有六株菌株都对一株CBPP菌株提供了部分但不完全的保护。第七株LC菌株（Mankefår 2833）是个例外：它很容易产生支原体血症，在这方面与SC菌株相似。与其他LC菌株一样，在交叉保护试验中，它对一株CBPP菌株仅提供了部分保护。Mankefår 2833菌株于1965年左右由布拉克从德国动物园的一只蛮羊（蛮羊属）中分离得到。Mankefår 2833产生支原体血症的能力使其能够用作交叉保护试验中的攻毒菌株。为了进行此类试验，上述九株支原体菌株的集合中又增加了来自早期研究的六株LC菌株。两株山羊SC菌株、一株CBPP菌株和九株LC菌株对Mankefår 2833提供了部分但不完全的保护。另外三株LC菌株提供的保护可能与同源菌株产生的保护一样强，但还需要进行验证实验。一株丝状支原体山羊亚种菌株对Mankefår 2833没有提供保护。
