通过小鼠支原体血症和交叉免疫试验对F38型及相关支原体进行的一项研究。

A study of F38-type and related mycoplasmas by mycoplasmaemia and cross-immunization tests in mice.

作者信息

Kanyi Kibe M, Smith G R

出版信息

J Hyg (Lond). 1984 Dec;93(3):465-73. doi: 10.1017/s0022172400065062.

DOI:10.1017/s0022172400065062

PMID:6392418

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2129469/

Abstract

In vivo methods were used to study the F38-type mycoplasma in parallel with related mycoplasmas. Three of five strains of 'bovine serogroup 7' with an unknown history of subculture produced mycoplasmaemia in mice inoculated intraperitoneally. A strain of 'bovine serogroup L' also produced mycoplasmaemia, but no evidence of similar ability could be found for single strains of Mycoplasma capricolum, M. equigenitalium and M. primatum, or for two strains of the F38-type mycoplasma. In cross-immunization tests a bovine serogroup 7 strain (NCTC 10133) and a strain ('Blenheim') of the SC (small colony) type of M. mycoides subsp. mycoides were used for the purpose of challenge. Cross-protection was described as 'complete' or 'partial', depending on whether it was as great as, or less than, that produced by homologous vaccine. Although strain NCTC 10133 protected strongly, possibly completely, against Blenheim, and Blenheim gave partial protection against NCTC 10133, challenge with NCTC 10133 and Blenheim gave strikingly different results. Thus (1) F38-type strains, M equigenitalium and M. primatum all gave partial cross-protection against NCTC 10133 but not against Blenheim, (2) NCTC 10133, unlike Blenheim, was seldom susceptible to partial cross-protection by LC (large colony) strains of M. mycoides subsp. mycoides, and (3) three SC strains - which would have protected completely against Blenheim - protected only partially against NCTC 10133. NCTC 10133 and Blenheim were similar, however, in that M. capricolum and M. mycoides subsp. capri failed to cross-protect against them both.

摘要

采用体内方法对F38型支原体及其相关支原体进行平行研究。五株“牛血清群7”菌株中有三株传代历史不明，经腹腔接种小鼠后产生了支原体血症。一株“牛血清群L”菌株也产生了支原体血症，但山羊支原体、马生殖道支原体、灵长类支原体的单株菌株以及两株F38型支原体均未发现有类似能力的证据。在交叉免疫试验中，使用一株牛血清群7菌株（NCTC 10133）和一株蕈状支原体丝状亚种SC（小菌落）型菌株（“布莱尼姆”）进行攻毒。交叉保护作用根据其是否与同源疫苗产生的保护作用相同或小于同源疫苗产生的保护作用，分为“完全”或“部分”。尽管菌株NCTC 10133对“布莱尼姆”有很强的、可能是完全的保护作用，而“布莱尼姆”对NCTC 10133有部分保护作用，但用NCTC 10133和“布莱尼姆”进行攻毒产生了截然不同的结果。因此，（1）F38型菌株、马生殖道支原体和灵长类支原体对NCTC 10133均有部分交叉保护作用，但对“布莱尼姆”没有；（2）与“布莱尼姆”不同，NCTC 10133很少受到丝状支原体丝状亚种LC（大菌落）菌株的部分交叉保护；（3）三株SC菌株——本应对“布莱尼姆”有完全保护作用——对NCTC 10133只有部分保护作用。然而，NCTC 10133和“布莱尼姆”也有相似之处，即山羊支原体和蕈状支原体山羊亚种对它们二者均无交叉保护作用。