[酿酒酵母D7菌株在环境遗传风险测定中的应用。2. 丙卡巴肼的遗传效应]

[Use of the D7 strain of S. cerevisiae in the determination of environmental genetic risk. 2. Genetic effects of procarbazine].

作者信息

Bronzetti G, Corsi C, Nieri R

出版信息

Boll Soc Ital Biol Sper. 1980 Jun 30;56(12):1322-8.

Abstract

Procarbazine was tested in vitro without and with S-10 fraction from mice liver (microsomal assay) using Saccharomyces cerevisiae strain D7, Salmonella typhimurium (strains TA 98, TA 100, TA 1535) and in vivo in Swiss albino mice (host mediated assay) using D7. Procarbazine without S-10 fraction was highly toxic and induced mitotic cross-over, gene conversion, and reverse mutation in D7. It had a toxic effect on all the Salmonella strains, but it did not induce any reverse mutations of the histidine mutants. In host-mediated assay using Saccharomyces, there was no genetic effect found in the indicator organism.

摘要

使用酿酒酵母菌株D7、鼠伤寒沙门氏菌（菌株TA 98、TA 100、TA 1535）在体外对丙卡巴肼进行了测试，测试时分别加入和未加入小鼠肝脏的S - 10组分（微粒体测定），并在体内使用D7对瑞士白化小鼠进行了测试（宿主介导测定）。未添加S - 10组分的丙卡巴肼具有高毒性，并在D7中诱导有丝分裂交换、基因转换和反向突变。它对所有沙门氏菌菌株都有毒性作用，但未诱导组氨酸突变体的任何反向突变。在使用酿酒酵母的宿主介导测定中，在指示生物中未发现遗传效应。