Koch Y, Rademacher K H
Can J Microbiol. 1980 Aug;26(8):965-70. doi: 10.1139/m80-164.
Candida albicans and Saccharomyces cerevisiae cells were examined by scanning scanning electron microscopy before and after extraction of the mannans of the cell wall. The surfaces of control cells were smooth; after mannan extraction they were rough and showed erosions which were particularly striking within the area of the scars. Helicase digested irregular holes through the cell wall within 20 min; these increased in size during an additional 40 min of digestion. These holes were not localized in or on the bud scars, which remained intact even after the long digestion period. The results were used to construct a model for yeast cell wall structure.
在提取细胞壁甘露聚糖之前和之后，通过扫描电子显微镜对白色念珠菌和酿酒酵母细胞进行了检查。对照细胞的表面是光滑的；甘露聚糖提取后，它们变得粗糙并出现侵蚀，在疤痕区域尤为明显。解旋酶在20分钟内消化出穿过细胞壁的不规则孔洞；在额外40分钟的消化过程中，这些孔洞尺寸增大。这些孔洞并不位于芽痕内或芽痕上，即使经过长时间消化，芽痕仍保持完整。这些结果被用于构建酵母细胞壁结构的模型。
