Kasai K, Aochi H, Shik S S, Yoshida Y
Nihon Naibunpi Gakkai Zasshi. 1980 Nov;56(11):1574-80. doi: 10.1507/endocrine1927.56.11_1574.
In the present paper, the authors studied the production and localization of human prolactin from normal human chorionic tissue and decidua in early pregnancy (from the 7th to the 9th week after last menstruation) obtained either by curettage or hysterectomy. Immunohistological investigation by the fluorescent antibody technique using human prolactin specific antiserum prepared by the immunization of rabbits revealed that syncytial trophoblast was a production site of prolactin. Though prolactin was recognized in compact layers of parietal decidua, it was concerned not with production but solely with deposition in intercellular space which was widened edematously with the existence of a collagen-like substance. By double staining method of the fluorescent antibody technique, prolactin could be differentiated with both hCG and hPL in syncytial trophoblast.
在本论文中，作者研究了通过刮宫术或子宫切除术获取的早期妊娠（末次月经后第7至9周）正常人类绒毛组织和蜕膜中人类催乳素的产生及定位。采用通过兔免疫制备的人类催乳素特异性抗血清，利用荧光抗体技术进行免疫组织学研究，结果显示合体滋养层是催乳素的产生部位。尽管在壁蜕膜的致密层中可识别出催乳素，但它与产生无关，仅与细胞间空间中的沉积有关，该细胞间空间因类胶原物质的存在而水肿性增宽。通过荧光抗体技术的双重染色方法，在合体滋养层中可将催乳素与hCG和hPL区分开来。
