Osada H
Nihon Naibunpi Gakkai Zasshi. 1976 Jan 20;52(1):36-53. doi: 10.1507/endocrine1927.52.1_36.
In 1936, Ehrhadt reported a prolactin-like substance which has been identified as a second protein hormone exists in extracted substance from the placenta. To date various reports have been published, but ther are still unsolved problems concerning the specifity of the substance, and its clinical significance is unclear. The purpose of this project was to purify and investigate the biochemical, immunological properties of HPL, and try to localize HPL secretion immunohistologically. The biological properties examined by intramuscular and intradermal pigeon crop sac reactions, both showed positive results. Biochemical properties were tested by 7.5% polyacrylamide disc electrophoresis and Free Fatty Acid mobilization. By these methods, the former showed a single band by electrophoresis with purified HPL 200 mug, and the latter showed a strong positive reaction both in vivo and in vitro. Immunological propert iese were examined by immunoelectrophoresis and the Ouchterlony method. In immunoelectrophoresis, the reaction of purified HPL 80 mug and anti-HPL serum resulted in a single strong zone. In constrast, anti-serum and HGH resulted in a weak zone of reaction. We confirmed that HGH cross reacts with our anti-HGH serum to HPL. The cross reaction of HGH with anti-HPL serum was reported by Josimovich et al, and other workers. In the Ouchterlony method, no precipitation line was detected between anti-HPL serum and 50-300 iu of HCG (PREGNYL). The results of investigating the localization of secritions of HPL immunohistologically, show that the specific fluorescence was limited to the strong reacting syncytial layer in the both the early chorionic villi of normal pregnancy and in the late stages of pregnancy. And the results of examining the localization with enzyme reactions with peroxdase from horseradish Type II, the reaction of benzidine brown was limited to the syncytial trophoblastic layer as in the case of FITC. These results suggests that the localization of the secretion of HPL exists in the syncytial layer of the chorionic villi.
1936年，埃尔哈德报告称，在从胎盘中提取的物质中存在一种催乳素样物质，该物质已被鉴定为第二种蛋白质激素。迄今为止，已有各种报告发表，但关于该物质的特异性仍存在未解决的问题，其临床意义也尚不清楚。本项目的目的是纯化并研究人胎盘催乳素（HPL）的生化、免疫学特性，并尝试通过免疫组织化学方法定位HPL的分泌部位。通过肌肉注射和皮内注射鸽嗉囊反应检测其生物学特性，结果均呈阳性。通过7.5%聚丙烯酰胺圆盘电泳和游离脂肪酸动员试验检测其生化特性。通过这些方法，前者用200微克纯化的HPL进行电泳显示出一条单一的条带，后者在体内和体外均显示出强烈的阳性反应。通过免疫电泳和双向免疫扩散法检测其免疫学特性。在免疫电泳中，80微克纯化的HPL与抗HPL血清反应产生一条单一的强反应带。相比之下，抗血清与人生长激素（HGH）反应产生一条弱反应带。我们证实HGH与我们的抗HGH血清对HPL有交叉反应。HGH与抗HPL血清的交叉反应已由约西莫维奇等人及其他研究人员报道。在双向免疫扩散法中，在抗HPL血清与50 - 300国际单位的人绒毛膜促性腺激素（HCG，商品名：普丽康）之间未检测到沉淀线。免疫组织化学研究HPL分泌定位的结果表明，特异性荧光仅限于正常妊娠早期绒毛和妊娠晚期绒毛膜的强反应合体层。用辣根过氧化物酶II型进行酶反应检测定位的结果，联苯胺棕色反应与异硫氰酸荧光素（FITC）情况一样，仅限于合体滋养层。这些结果表明，HPL的分泌定位存在于绒毛膜绒毛的合体层。
