Goud B, Antoine J C, Gonatas N K, Stieber A, Avrameas S
Immunology. 1980 Dec;41(4):833-48.
Immunoenzymatic techniques were used to study antigen binding and endocytosis by lymph node cells of rats immunized against horseradish peroxidase, hen ovalbumin and rabbit IgG. The number of antigen-binding cells varied and depended on the type of antigen used, the time after immunization, and was higher after a booster injection. In secondary responses (4 days after booster), about 80% of antigen-binding cells were proplasmocytes and plasmocytes; by a double staining procedure it was found that 82% of these cells bore in addition to surface antigen, specific intracytoplasmic antibody as well. About 20% of antigen-binding cells were small and medium lymphocytes which did not contain detectable intracytoplasmic antibody. For ultrastructural studies of the endocytosis, peroxidase was used as the antigen. This antigen was found in cytoplasmic compartments which consisted of vesicles, cisternae and large round bodies (lysosomes?) often located near the Golgi apparatus. However, the cisternae of the Golgi apparatus, involved in the synthesis of specific antibody were not sites of retrieval of endocytosed antigen. The effect of endocytosis of antigen on the secretion and synthesis of antibody was studied by the local haemolysis plaque assay and biosynthetic labelling. No change was detected in antibody secretion and synthesis as a result of antigen endocytosis.
采用免疫酶技术研究了用辣根过氧化物酶、鸡卵白蛋白和兔IgG免疫的大鼠淋巴结细胞的抗原结合和内吞作用。抗原结合细胞的数量各不相同，取决于所用抗原的类型、免疫后的时间，且在加强注射后数量更多。在二次反应中（加强注射后4天），约80%的抗原结合细胞是前浆细胞和浆细胞；通过双重染色程序发现，这些细胞中82%除表面抗原有特异性胞浆内抗体外。约20%的抗原结合细胞是小淋巴细胞和中淋巴细胞，它们不含可检测到的胞浆内抗体。为了对内吞作用进行超微结构研究，以过氧化物酶作为抗原。该抗原存在于由囊泡、池和通常位于高尔基体附近的大圆形体（溶酶体？）组成的细胞质区室中。然而，参与特异性抗体合成的高尔基体池并不是内吞抗原回收的部位。通过局部溶血空斑试验和生物合成标记研究了抗原内吞对抗体分泌和合成的影响。未检测到由于抗原内吞导致的抗体分泌和合成的变化。
