Sinski J T, Van Avermaete D, Kelley L M
J Clin Microbiol. 1981 Jan;13(1):62-5. doi: 10.1128/jcm.13.1.62-65.1981.
Differential identification of Trichophyton rubrum and Trichophyton mentagrophytes was evaluated by using the standard in vitro hair perforation test as a criterion for identification and comparing its effectiveness with that of nine other tests used to differentiate these two taxa. Correlation and multiple regression analysis showed that no single test or combination of these tests was able to make the differentiation as effectively as the standard in vitro hair perforation test. Using relative error analysis, we suggest an identification scheme based upon selective utilization of potato-carrot agar for sporulation and the standard in vitro hair perforation test. Using potato-carrot agar for development of typical microconidia on each of the nonsporulating primary isolates and subsequently performing the standard in vitro hair perforation test only on those isolates that failed to produce spores on potato-carrot medium, we showed that this combination scheme provides more expedient results, without any sacrifice in reliability, than the standard in vitro hair perforation test alone.
通过使用标准的体外毛发穿孔试验作为鉴定标准，并将其与用于区分这两个分类单元的其他九种试验的有效性进行比较，评估红色毛癣菌和须癣毛癣菌的鉴别。相关性和多元回归分析表明，没有单一试验或这些试验的组合能够像标准的体外毛发穿孔试验那样有效地进行区分。使用相对误差分析，我们提出了一种基于选择性利用马铃薯-胡萝卜琼脂进行孢子形成和标准体外毛发穿孔试验的鉴定方案。在每个不产孢的原始分离株上使用马铃薯-胡萝卜琼脂培养典型的小分生孢子，随后仅对那些在马铃薯-胡萝卜培养基上未能产生孢子的分离株进行标准的体外毛发穿孔试验，我们表明，与单独的标准体外毛发穿孔试验相比，这种组合方案能提供更便捷的结果，且在可靠性上没有任何牺牲。
