标准血清的冷冻保存。
The cryopreservation of standard sera.
作者信息
Tosswill J H, Ridley D S
出版信息
J Clin Pathol. 1981 Jan;34(1):76-7. doi: 10.1136/jcp.34.1.76.
To determine the storage temperature needed for the preservation of standard sera for serodiagnostic tests, groups of sera were tested before and after storage at -70 degrees C and -20 degrees C for periods of up to five years. The temperature required depended on the nature of the test, but no definite advantage was found in storage at -70 degrees C. For complement fixation this temperature was inadequate and greatly inferior to liquid nitrogen. For immunofluorescence it had no apparent advantage over a temperature of -20 degrees C, and for some other tests it was no more than marginally superior.
为确定用于血清学诊断试验的标准血清保存所需的储存温度，将血清分组，在-70℃和-20℃下储存长达五年前后进行检测。所需温度取决于试验性质，但发现在-70℃储存并无明确优势。对于补体结合试验，该温度并不适宜，且远不如液氮。对于免疫荧光试验，与-20℃相比它并无明显优势，而对于其他一些试验，它仅略占优势。
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