Weber T H, Mäkinen T, Welin M G
J Clin Lab Immunol. 1980 Jul;4(1):47-8.
The activity of LIF, produced in vitro by sensitized lymphocytes exposed to the appropriate antigens, is readily abolished in the simultaneous presence of heparin and thrombin. Heparin or thrombin alone are without significant effects. Adding AT III together with heparin and thrombin restores the LIF activity. We suggest that the migrating leucocytes on their cell membranes have receptors, composed of AT III-like molecules, which are blocked or destroyed, by forming complexes with heparin and thrombin. However, the heparin-thrombin complex will preferentially interact with AT III added to the culture medium, thus leaving the cellular receptors free to interact with LIF.
致敏淋巴细胞在体外接触适当抗原时产生的白血病抑制因子（LIF）活性，在肝素和凝血酶同时存在的情况下会迅速被消除。单独的肝素或凝血酶没有显著影响。将抗凝血酶III（AT III）与肝素和凝血酶一起添加可恢复LIF活性。我们认为，迁移的白细胞在其细胞膜上具有由AT III样分子组成的受体，这些受体通过与肝素和凝血酶形成复合物而被阻断或破坏。然而，肝素-凝血酶复合物将优先与添加到培养基中的AT III相互作用，从而使细胞受体能够自由地与LIF相互作用。
