Goto K, Sato H
Tohoku J Exp Med. 1980 Aug;131(4):399-407. doi: 10.1620/tjem.131.399.
A mixture of an ethanolic solution of phospholipid and water was sonicated, kept at 50 degrees C for 30 min, and then observed by a phase-contrast microscope. A large number of cell-sized, single-layered liposomes thus obtained were different in shape and thickness from the liposomes prepared by conventional methods. Fixation with La(NO3)3 and KMnO4 stabilized the liposomes. As a result they remained unchanged in the organic solvents used for dehydration and embedding. Ultrathin sections of the liposomes could, therefore, be observed with an electron microscope. The nature of these liposomes was discussed in relation to the structure and function of the cell membrane.
将磷脂乙醇溶液和水的混合物进行超声处理，在50摄氏度下保持30分钟，然后用相差显微镜观察。由此获得的大量细胞大小的单层脂质体在形状和厚度上与通过传统方法制备的脂质体不同。用硝酸镧和高锰酸钾固定可使脂质体稳定。结果，它们在用于脱水和包埋的有机溶剂中保持不变。因此，可以用电子显微镜观察脂质体的超薄切片。结合细胞膜的结构和功能对这些脂质体的性质进行了讨论。
