Shew R L, Deamer D W
Biochim Biophys Acta. 1985 Jun 11;816(1):1-8. doi: 10.1016/0005-2736(85)90386-4.
Hemoglobin and alkaline phosphatase were each encapsulated in phosphatidylcholine liposomes using a dehydration-rehydration cycle for liposome formation. In this method, liposomes prepared by sonication are mixed in aqueous solution with the solute desired to be encapsulated and the mixture is dried under nitrogen in a rotating flask. As the sample is dehydrated, the liposomes fuse to form a multilamellar film that effectively sandwiches the solute molecules. Upon rehydration, large liposomes are produced which have encapsulated a significant fraction of the solute. The optimal mass ratio of lipid to solute is approx. 1:2 to 1:3. This method has potential application in large-scale liposome production, since it depends only on a controlled drying and rehydration process, and does not require extensive use of organic solvents, detergents, or dialysis systems.
血红蛋白和碱性磷酸酶分别通过脱水-复水循环法包裹于磷脂酰胆碱脂质体中以形成脂质体。在该方法中，通过超声处理制备的脂质体与期望包裹的溶质在水溶液中混合，然后在旋转烧瓶中于氮气保护下干燥。随着样品脱水，脂质体融合形成多层膜，有效地将溶质分子夹在中间。复水后，会产生包裹了相当一部分溶质的大脂质体。脂质与溶质的最佳质量比约为1:2至1:3。该方法在大规模脂质体生产中具有潜在应用价值，因为它仅依赖于可控的干燥和复水过程，且无需大量使用有机溶剂、去污剂或透析系统。
