Knowles D M, Jakobiec F A
Arch Ophthalmol. 1981 Feb;99(2):309-16. doi: 10.1001/archopht.1981.03930010311020.
T lymphocytes may be identified by two comparatively simple techniques: sheep erythrocyte (E) rosette formation and cytochemically demonstrable acid alpha-naphthyl acetate esterase (ANAE) activity. We tested the quantitative determination of T cells in 17 ocular lymphoid tumors as an indirect method for characterizing their clonality. Six lesions containing greater than 40% T cells (47% to 73%; mean, 62%) were shown to be polyclonal proliferations and were classified as pseudolymphomas by histologic criteria. Seven lesions containing less than 30% T cells (3% to 20%; mean, 10%) were shown to be monoclonal B-cell proliferations and were classified as malignant lymphomas by histologic criteria. Only two lesions containing between 30% and 40% T cells could not be unequivocally assigned to the monoclonal or polyclonal category solely based on the percentage of T cells. In the final two lesions, the tissue specimen was too small to allow a full panel of immunologic studies; both tumors showed a predominance of T cells consistent with their benign histologic features.
T淋巴细胞可用两种相对简单的技术来识别：绵羊红细胞（E）玫瑰花结形成以及细胞化学可显示的酸性α-萘乙酸酯酶（ANAE）活性。我们检测了17例眼淋巴肿瘤中T细胞的定量测定，作为表征其克隆性的一种间接方法。6个病变中T细胞含量超过40%（47%至73%；平均62%），显示为多克隆增殖，根据组织学标准被归类为假淋巴瘤。7个病变中T细胞含量低于30%（3%至20%；平均10%），显示为单克隆B细胞增殖，根据组织学标准被归类为恶性淋巴瘤。只有2个病变中T细胞含量在30%至40%之间，仅根据T细胞百分比无法明确归入单克隆或多克隆类别。在最后2个病变中，组织标本太小，无法进行全面的免疫学研究；这两个肿瘤均显示T细胞占优势，与其良性组织学特征相符。
