Sundström C, Nilsson K, Ranki A, Häyry P
Scand J Haematol. 1978 Jul;21(1):47-59. doi: 10.1111/j.1600-0609.1978.tb02494.x.
We have previously shown that non-proliferating human T- but not B-lymphocytes contain demonstrable amounts of acid alpha-naphthyl acetate esterase (ANAE). The usefulness of this histochemical marker for the diagnosis and classification of malignant lymphoid tumors was investigated by use of a panel of established normal and malignant human haematopoietic cell lines and fresh biopsy cells from malignant lymphomas and myelomas. The results showed that not only the T-cell derived acute leukaemia lines, but also histiocytic lymphoma and myeloma lines and some of the lymphoma (Burkitt and lymphocytic) and non-neoplastic lymphoblastoid cell lines with B-cell surface markers expressed strong ANAE reactivity. Some but not all of the immunoglobulin producing myeloma and lymphocytic lymphoma biopsies were ANAE-positive. Inhibition experiments with sodium fluoride and E-600 demonstrated that although the T-lymphocyte specific esterase is predominantly of 'A'-type, the malignant lines contain also non-specific 'B' esterase and pseudocholinesterase. As the presence of the various esterases did not demonstrate any specific distribution pattern among he haematopoietic cell lines of different origin, we concluded that the ANAE marker is no longer T-specific when malignant lymphoid cells are considered, and that the usefulness of this marker in routine diagnostic work therefore is limited.
我们之前已经表明，非增殖性人类T淋巴细胞而非B淋巴细胞含有可检测到的酸性α-萘乙酸酯酶（ANAE）。通过使用一组已建立的正常和恶性人类造血细胞系以及来自恶性淋巴瘤和骨髓瘤的新鲜活检细胞，研究了这种组织化学标记物在恶性淋巴瘤诊断和分类中的实用性。结果显示，不仅T细胞来源的急性白血病细胞系，而且组织细胞淋巴瘤和骨髓瘤细胞系以及一些具有B细胞表面标志物的淋巴瘤（伯基特淋巴瘤和淋巴细胞淋巴瘤）和非肿瘤性淋巴母细胞系都表现出强烈的ANAE反应性。一些但并非所有产生免疫球蛋白的骨髓瘤和淋巴细胞淋巴瘤活检标本呈ANAE阳性。用氟化钠和E - 600进行的抑制实验表明，虽然T淋巴细胞特异性酯酶主要是“A”型，但恶性细胞系中也含有非特异性“B”酯酶和假性胆碱酯酶。由于各种酯酶的存在在不同来源的造血细胞系中未显示出任何特定的分布模式，我们得出结论，当考虑恶性淋巴细胞时，ANAE标记物不再是T特异性的，因此该标记物在常规诊断工作中的实用性有限。
