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胰岛素对大鼠肝细胞电泳迁移率的影响。

The effect of insulin on the electrophoretic mobility of rat hepatocytes.

作者信息

Delpech C B, Perry M C, Plummer D T

出版信息

Experientia. 1980 Dec 15;36(12):1376-8. doi: 10.1007/BF01960106.

DOI:10.1007/BF01960106
PMID:7009195
Abstract

The addition of insulin (10 microU) to a suspension of isolated hepatocytes in Krebs-Ringer bicarbonate solution, causes an increase in the negative electrophoretic mobility of the cells from - 1.68 micrometer sec-1 V-1 cm to 2.26 micrometer sec-1 V-1 cm. This observation supports the findings by other workers that the binding of insulin to its receptor leads to a marked change in the membrane.

摘要

在克雷布斯-林格碳酸氢盐溶液中向分离的肝细胞悬液中添加胰岛素(10微单位),会使细胞的负电泳迁移率从-1.68微米·秒⁻¹·伏特⁻¹·厘米增加到-2.26微米·秒⁻¹·伏特⁻¹·厘米。这一观察结果支持了其他研究人员的发现,即胰岛素与其受体的结合会导致细胞膜发生显著变化。

相似文献

1
The effect of insulin on the electrophoretic mobility of rat hepatocytes.胰岛素对大鼠肝细胞电泳迁移率的影响。
Experientia. 1980 Dec 15;36(12):1376-8. doi: 10.1007/BF01960106.
2
Molecules at the external nuclear surface. Sialic acid of nuclear membranes and electrophoretic mobility of isolated nuclei and nucleoli.核外表面的分子。核膜的唾液酸以及分离的细胞核和核仁的电泳迁移率。
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3
Mitochondrial autonomy. Sialic acid residues on the surface of isolated rat cerebral cortex and liver mitochondria.线粒体自主性。分离的大鼠大脑皮层和肝脏线粒体表面的唾液酸残基。
J Cell Biol. 1972 Oct;55(1):147-60. doi: 10.1083/jcb.55.1.147.
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Studies on the in vitro effects of insulin on glycogen synthesis and ultrastructure in isolated rat liver hepatocytes.胰岛素对离体大鼠肝脏肝细胞糖原合成及超微结构的体外作用研究。
Biochem Biophys Res Commun. 1973 Aug 6;53(3):937-43. doi: 10.1016/0006-291x(73)90182-4.
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Effects of concanavalin A on the isolated perfused rat liver.
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6
Activation and glucagon regulation of mitogen-activated protein kinases (MAPK) by insulin and epidermal growth factor in cultured rat and human hepatocytes.胰岛素和表皮生长因子对培养的大鼠及人肝细胞中丝裂原活化蛋白激酶(MAPK)的激活作用及胰高血糖素调节
Cell Biochem Funct. 1998 Jun;16(2):77-85. doi: 10.1002/(SICI)1099-0844(199806)16:2<77::AID-CBF769>3.0.CO;2-U.
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Binding of concanavalin A to isolated hepatocytes and its effect on uptake and degradation of asialo-fetuin by the cells.伴刀豆球蛋白A与分离的肝细胞的结合及其对细胞摄取和降解去唾液酸胎球蛋白的影响。
Biochem J. 1980 Sep 15;190(3):697-703. doi: 10.1042/bj1900697.
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The electrophoretic behaviour of mitochondria from kidney and liver of rats.大鼠肾脏和肝脏中线粒体的电泳行为。
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Concanavalin A can trap insulin and increase insulin internalization into cells cultured in monolayer.刀豆球蛋白A能够捕获胰岛素并增加胰岛素向单层培养细胞内的内化作用。
Biochem Biophys Res Commun. 1984 Jul 18;122(1):204-11. doi: 10.1016/0006-291x(84)90460-1.

本文引用的文献

1
Accumulation of amino acids in muscle of perfused rat heart. Effect of insulin.灌注大鼠心脏肌肉中氨基酸的积累。胰岛素的作用。
Biochem J. 1965 Oct;97(1):257-71. doi: 10.1042/bj0970257.
2
Effect of insulin upon resting electrical potential of adipose tissue.胰岛素对脂肪组织静息电位的影响。
Proc Soc Exp Biol Med. 1962 Jul;110:590-5. doi: 10.3181/00379727-110-27588.
3
Effect of insulin on membrane potential and potassium content of rat muscle.胰岛素对大鼠肌肉膜电位和钾含量的影响。
Am J Physiol. 1959 Sep;197:515-23. doi: 10.1152/ajplegacy.1959.197.3.515.
4
Mediated (nonactive) transport of glucose in mammalian cells and its regulation.哺乳动物细胞中葡萄糖的介导(非主动)转运及其调节。
J Gen Physiol. 1968 Jul;52(1):296Suppl-318s.
5
High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.大鼠肝脏实质细胞分离的高效制备:生化与精细结构研究
J Cell Biol. 1969 Dec;43(3):506-20. doi: 10.1083/jcb.43.3.506.
6
Effect of insulin upon the sodium pump in frog skeletal muscle.胰岛素对青蛙骨骼肌钠泵的作用。
J Physiol. 1973 Jul;232(1):23-45. doi: 10.1113/jphysiol.1973.sp010255.
7
Insulin-like activity of concanavalin A and wheat germ agglutinin--direct interactions with insulin receptors.伴刀豆球蛋白A和麦胚凝集素的胰岛素样活性——与胰岛素受体的直接相互作用。
Proc Natl Acad Sci U S A. 1973 Feb;70(2):485-9. doi: 10.1073/pnas.70.2.485.
8
Guanosine 3':5'-cyclic monophosphate and the action of insulin and acetylcholine.3':5'-环磷酸鸟苷以及胰岛素与乙酰胆碱的作用
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2443-7. doi: 10.1073/pnas.70.8.2443.
9
Insulin receptors.胰岛素受体
Annu Rev Med. 1973;24:233-40. doi: 10.1146/annurev.me.24.020173.001313.
10
Insulin-stimulated plasma membranes from rat adipocytes: their physiological and physicochemical properties.来自大鼠脂肪细胞的胰岛素刺激质膜:其生理和物理化学性质。
Biochim Biophys Acta. 1972 Oct 23;288(1):27-42. doi: 10.1016/0005-2736(72)90220-9.