• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大鼠肾脏和肝脏中线粒体的电泳行为。

The electrophoretic behaviour of mitochondria from kidney and liver of rats.

作者信息

Plummer D T

出版信息

Biochem J. 1965 Sep;96(3):729-32. doi: 10.1042/bj0960729.

DOI:10.1042/bj0960729
PMID:5867784
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1207210/
Abstract
  1. The electrophoretic mobility values for mitochondria prepared from rat kidney and liver measured in 0.125m-potassium chloride-0.02m-tris, pH7.4, are: -0.78(s.e.m.+/-0.02)mu/sec./v/cm. (29 experiments) and -1.06(s.e.m.+/-0.01) mu/sec./v/cm. (21 experiments) respectively. 2. These mobility values are unaffected by washing and spontaneous swelling at 25 degrees , indicating a stable electrokinetic surface. 3. The mobility of rat-kidney mitochondria is unaffected by thyroxine-induced swelling, or by the state of hydration of the rat. 4. pH-mobility curves show similar surface ionogenic groups for kidney and liver mitochondria; their isoelectric points are pH3.9 and pH4.4 respectively.
摘要
  1. 在0.125m氯化钾 - 0.02m三羟甲基氨基甲烷（Tris），pH7.4条件下测得的从大鼠肾脏和肝脏制备的线粒体的电泳迁移率值分别为：-0.78（标准误±0.02）μm/秒/伏/厘米（29次实验）和 -1.06（标准误±0.01）μm/秒/伏/厘米（21次实验）。2. 这些迁移率值不受25℃下洗涤和自发肿胀的影响，表明电动表面稳定。3. 大鼠肾脏线粒体的迁移率不受甲状腺素诱导的肿胀或大鼠水合状态的影响。4. pH - 迁移率曲线显示肾脏和肝脏线粒体具有相似的表面离子基团；它们的等电点分别为pH3.9和pH4.4。

相似文献

1
The electrophoretic behaviour of mitochondria from kidney and liver of rats.大鼠肾脏和肝脏中线粒体的电泳行为。
Biochem J. 1965 Sep;96(3):729-32. doi: 10.1042/bj0960729.
2
Mitochondrial autonomy. Sialic acid residues on the surface of isolated rat cerebral cortex and liver mitochondria.线粒体自主性。分离的大鼠大脑皮层和肝脏线粒体表面的唾液酸残基。
J Cell Biol. 1972 Oct;55(1):147-60. doi: 10.1083/jcb.55.1.147.
3
Molecules at the external nuclear surface. Sialic acid of nuclear membranes and electrophoretic mobility of isolated nuclei and nucleoli.核外表面的分子。核膜的唾液酸以及分离的细胞核和核仁的电泳迁移率。
J Cell Biol. 1973 Dec;59(3):601-14. doi: 10.1083/jcb.59.3.601.
4
Two forms of aspartate aminotransferase in rat liver and kidney mitochondria.大鼠肝脏和肾脏线粒体中的两种天冬氨酸氨基转移酶形式。
Biochem J. 1968 Jul;108(4):619-24. doi: 10.1042/bj1080619.
5
Effect of lanthanum ion on platelet electrophoretic mobility.镧离子对血小板电泳迁移率的影响。
Haematologia (Budap). 1977;11(1-2):155-61.
6
Conditions for activity of glutaminase in kidney mitochondria.肾脏线粒体中谷氨酰胺酶的活性条件。
Biochem J. 1970 Jun;118(2):265-74. doi: 10.1042/bj1180265.
7
Characteristic inhibition of in vitro swelling of purified rat liver mitochondria induced by Ca2+ or thyroxine by feeding rats a low casein diet.
J Nutr. 1976 Nov;106(11):1637-42. doi: 10.1093/jn/106.11.1637.
8
The effect of insulin on the electrophoretic mobility of rat hepatocytes.胰岛素对大鼠肝细胞电泳迁移率的影响。
Experientia. 1980 Dec 15;36(12):1376-8. doi: 10.1007/BF01960106.
9
Separation and properties of beta-galactosidase, beta-glucosidase, beta-glucuronidase and N-acetyl-beta-glucosaminidase from rat kidney.大鼠肾脏中β-半乳糖苷酶、β-葡萄糖苷酶、β-葡萄糖醛酸酶和N-乙酰-β-葡萄糖胺酶的分离及性质
Biochem J. 1967 Feb;102(2):525-32. doi: 10.1042/bj1020525.
10
The ionogenic nature of the secretory-granule membrane. Electrokinetic properties of isolated chromaffin granules.分泌颗粒膜的离子ogenic性质。分离的嗜铬颗粒的电动性质。
Biochem J. 1972 Dec;130(3):825-32. doi: 10.1042/bj1300825.

引用本文的文献

1
Capillary isoelectric focusing of individual mitochondria.单个线粒体的毛细管等电聚焦
Anal Chem. 2011 Jan 15;83(2):612-8. doi: 10.1021/ac102712r. Epub 2010 Dec 30.
2
Fast determination of mitochondria electrophoretic mobility using micro free-flow electrophoresis.使用微流控自由电泳快速测定线粒体电泳迁移率。
Anal Chem. 2009 Nov 15;81(22):9267-73. doi: 10.1021/ac901508x.
3
Effect of pH and different substrates on the electrokinetic properties of (Na+, K+)-ATPase vesicles.pH值和不同底物对（钠钾）-ATP酶囊泡电动性质的影响。
Biophys Struct Mech. 1983;9(3):193-206. doi: 10.1007/BF00537816.
4
An electron microscopic study of structural changes during the large amplitude swelling and contraction of isolated beef-heart mitochondria.对分离的牛心线粒体大幅度肿胀和收缩过程中结构变化的电子显微镜研究。
Z Zellforsch Mikrosk Anat. 1967;80(2):205-13. doi: 10.1007/BF00337456.
5
A new method for the preparation of rat liver lysosomes. Separation of cell organelles of rat liver by carrier-free continuous electrophoresis.一种制备大鼠肝脏溶酶体的新方法。通过无载体连续电泳分离大鼠肝脏的细胞器。
J Cell Biol. 1970 Sep;46(3):576-91. doi: 10.1083/jcb.46.3.576.

本文引用的文献

1
The effects of progressive nutritional hypoproteinaemia of the extracellular-fluid phase and plasma colloid osmotic pressure in rats.细胞外液相进行性营养性低蛋白血症及血浆胶体渗透压对大鼠的影响。
Biochem J. 1948;43(3):444-53.
2
ON THE MECHANISM OF ACTION OF THE ANTIDIURETIC HORMONE (VASOPRESSIN).抗利尿激素（血管加压素）的作用机制
Proc Natl Acad Sci U S A. 1960 Oct;46(10):1273-7. doi: 10.1073/pnas.46.10.1273.
3
Some physical investigations of the behaviour of bacterial surfaces. V. The variation of the surface structure of Streptococcus pyogenes during growth.细菌表面行为的一些物理研究。V. 化脓性链球菌生长过程中表面结构的变化。
Biochim Biophys Acta. 1962 Jul 16;60:595-603. doi: 10.1016/0006-3002(62)90878-8.
4
Water uptake and extrusion by mitochondria in relation to oxidative phosphorylation.线粒体的水摄取与排出及其与氧化磷酸化的关系
Physiol Rev. 1962 Jul;42:467-517. doi: 10.1152/physrev.1962.42.3.467.
5
Effect of oxytocin, vasopressin, and other disulfide hormones on uptake and extrusion of water by mitochondria.催产素、加压素及其他二硫键激素对线粒体水摄取与排出的影响。
Proc Natl Acad Sci U S A. 1961 Dec 15;47(12):1929-36. doi: 10.1073/pnas.47.12.1929.
6
The relationship of internal conductance and membrane capacity to mitochondrial volume.内部电导率和膜电容与线粒体体积的关系。
J Biophys Biochem Cytol. 1960 Jul;7(4):603-12. doi: 10.1083/jcb.7.4.603.
7
CHANGES IN SULFHYDRYL GROUPS OF RAT LIVER MITOCHONDRIA DURING SWELLING AND CONTRACTION.大鼠肝脏线粒体肿胀和收缩过程中巯基的变化
J Biol Chem. 1964 Jun;239:2083-9.
8
THE EFFECTS OF MAMMALIAN POSTERIOR LOBE HORMONES ON THE SWELLING OF LIVER AND KIDNEY MITOCHONDRIA, IN THE RAT AND THE DOG.
Biochim Biophys Acta. 1963 Aug 13;74:519-24. doi: 10.1016/0006-3002(63)91394-5.
9
An electrophoretic study of suspensions of intact mitochondria and fragments of mitochondrial membranes.完整线粒体及线粒体膜片段悬浮液的电泳研究。
Biochim Biophys Acta. 1961 Jun 24;50:213-23. doi: 10.1016/0006-3002(61)90319-5.
10
An improved microelectrophoresis apparatus and technique for studying biological cell surfaces.一种用于研究生物细胞表面的改进型微电泳装置及技术。
Anal Biochem. 1960 Dec 30;1:478-85. doi: 10.1016/0003-2697(60)90060-9.