大鼠肾脏和肝脏中线粒体的电泳行为。
The electrophoretic behaviour of mitochondria from kidney and liver of rats.
作者信息
Plummer D T
出版信息
Biochem J. 1965 Sep;96(3):729-32. doi: 10.1042/bj0960729.
Abstract
- The electrophoretic mobility values for mitochondria prepared from rat kidney and liver measured in 0.125m-potassium chloride-0.02m-tris, pH7.4, are: -0.78(s.e.m.+/-0.02)mu/sec./v/cm. (29 experiments) and -1.06(s.e.m.+/-0.01) mu/sec./v/cm. (21 experiments) respectively. 2. These mobility values are unaffected by washing and spontaneous swelling at 25 degrees , indicating a stable electrokinetic surface. 3. The mobility of rat-kidney mitochondria is unaffected by thyroxine-induced swelling, or by the state of hydration of the rat. 4. pH-mobility curves show similar surface ionogenic groups for kidney and liver mitochondria; their isoelectric points are pH3.9 and pH4.4 respectively.
摘要
- 在0.125m氯化钾 - 0.02m三羟甲基氨基甲烷（Tris），pH7.4条件下测得的从大鼠肾脏和肝脏制备的线粒体的电泳迁移率值分别为：-0.78（标准误±0.02）μm/秒/伏/厘米（29次实验）和 -1.06（标准误±0.01）μm/秒/伏/厘米（21次实验）。2. 这些迁移率值不受25℃下洗涤和自发肿胀的影响，表明电动表面稳定。3. 大鼠肾脏线粒体的迁移率不受甲状腺素诱导的肿胀或大鼠水合状态的影响。4. pH - 迁移率曲线显示肾脏和肝脏线粒体具有相似的表面离子基团；它们的等电点分别为pH3.9和pH4.4。
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