• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

固定化亚基可作为亲和吸附剂用于纯化寡聚酶：固相支持物上杂交的实用性。

Immobilized subunits can function as an affinity sorbent to purify oligomeric enzymes: the usefulness of hybridization on the solid support.

作者信息

Muronetz V I, Ashmarina L I, Nagradova N K

出版信息

Experientia. 1981 Jan 15;37(1):15-6. doi: 10.1007/BF01965542.

DOI:10.1007/BF01965542
PMID:7009196
Abstract

Immobilized dimers of yeast glyceraldehyde-3-phosphate dehydrogenase covalently bound to sepharose were shown to form hybrids with soluble dimers of the homologous enzymes present in crude tissue extracts (rats skeletal muscle, rat, rabbit and bovine hearts, rat liver, rat brain). Immobilized hybrid tetramers were then dissociated to form purified soluble enzymes.

摘要

已证明，与琼脂糖共价结合的酵母甘油醛-3-磷酸脱氢酶固定化二聚体可与粗组织提取物（大鼠骨骼肌、大鼠、兔和牛心脏、大鼠肝脏、大鼠脑）中存在的同源酶的可溶性二聚体形成杂交体。然后将固定化杂交四聚体解离以形成纯化的可溶性酶。

相似文献

1
Immobilized subunits can function as an affinity sorbent to purify oligomeric enzymes: the usefulness of hybridization on the solid support.固定化亚基可作为亲和吸附剂用于纯化寡聚酶：固相支持物上杂交的实用性。
Experientia. 1981 Jan 15;37(1):15-6. doi: 10.1007/BF01965542.
2
Immobilized hybrids of glyceraldehyde-3-phosphate dehydrogenase.固定化的3-磷酸甘油醛脱氢酶杂交体
Biochim Biophys Acta. 1978 Jul 7;525(1):291-4. doi: 10.1016/0005-2744(78)90223-1.
3
[Use of immobilization for the study of glyceraldehyde 3-phosphate dehydrogenase. Immobilized dimers of the enzyme].[使用固定化技术研究3-磷酸甘油醛脱氢酶。该酶的固定化二聚体]
Biokhimiia. 1982 Jan;47(1):3-12.
4
[Use of immobilization for investigation of glyceraldehyde 3-phosphate dehydrogenase. Immobilized tetramers].[利用固定化技术研究3-磷酸甘油醛脱氢酶。固定化四聚体]
Biokhimiia. 1981 Oct;46(10):1731-9.
5
Study of subunit interactions in immobilized D-glyceraldehyde-3-phosphate dehydrogenase.固定化3-磷酸甘油醛脱氢酶中亚基相互作用的研究。
Biochim Biophys Acta. 1980 Jun 13;613(2):292-308. doi: 10.1016/0005-2744(80)90084-4.
6
[Possible use of the hybridization of oligomeric enzymes in biomedical research].
Vestn Akad Med Nauk SSSR. 1986(8):32-40.
7
Use of differently immobilized nucleotides for binding NAD+-dependent dehydrogenases.使用不同固定化核苷酸结合NAD+依赖性脱氢酶。
Methods Enzymol. 1980;66:192-208. doi: 10.1016/0076-6879(80)66459-3.
8
[Phosphorylation of glyceraldehyde-3-phosphate dehydrogenase].
Biokhimiia. 1993 Apr;58(4):636-47.
9
[Immobilization of glyceraldehyde-3-phosphate dehydrogenase by non-covalent binding to specific antibodies and Fab-fragments coupled to Sepharose].通过与结合到琼脂糖凝胶上的特异性抗体和Fab片段非共价结合来固定3-磷酸甘油醛脱氢酶
Biokhimiia. 1978 Jul;43(7):1277-84.
10
Quantitative studies of ion-exchange and affinity elution chromatography of enzymes.酶的离子交换和亲和洗脱色谱法的定量研究。
Anal Biochem. 1981 Jun;114(1):8-18. doi: 10.1016/0003-2697(81)90443-7.

本文引用的文献

1
Yeast Glyceraldehyde-3-phosphate dehydrogenase. I. Electrophoresis of fractions precipitated by nucleic acid.酵母3-磷酸甘油醛脱氢酶。I. 核酸沉淀组分的电泳分析
J Biol Chem. 1953 Feb;200(2):471-8.
2
Immobilized dimers of D-glyceraldehyde-3-phosphate dehydrogenase.
FEBS Lett. 1974 Dec 15;49(2):242-5. doi: 10.1016/0014-5793(74)80521-1.
3
Influence of substrates on the dissociation of rabbit muscle D-glyceraldehyde 3-phosphate dehydrogenase.底物对兔肌肉3-磷酸甘油醛脱氢酶解离的影响。
Biochemistry. 1969 Feb;8(2):594-602. doi: 10.1021/bi00830a020.
4
Half-of-the-sites reactivity of rat skeletal muscle D-glyceraldehyde-3-phosphate dehydrogenase.
Biochim Biophys Acta. 1978 May 11;524(1):15-25. doi: 10.1016/0005-2744(78)90098-0.
5
A convenient method for the estimation of Sepharose-bound protein.
Anal Biochem. 1977 Dec;83(2):778-81. doi: 10.1016/0003-2697(77)90085-9.
6
Dissociation and reassociation of immobilized porphobilinogen synthase: use of immobilized subunits for enzyme isolation.固定化胆色素原合酶的解离与重新结合：利用固定化亚基进行酶的分离
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1383-7. doi: 10.1073/pnas.74.4.1383.
7
Cold inactivation of glyceraldehyde-phosphate dehydrogenase from rat skeletal muscle.大鼠骨骼肌磷酸甘油醛脱氢酶的冷失活
Biochim Biophys Acta. 1975 Jan 23;377(1):15-25. doi: 10.1016/0005-2744(75)90281-8.
8
Immobilized hybrids of glyceraldehyde-3-phosphate dehydrogenase.固定化的3-磷酸甘油醛脱氢酶杂交体
Biochim Biophys Acta. 1978 Jul 7;525(1):291-4. doi: 10.1016/0005-2744(78)90223-1.