固定化亚基可作为亲和吸附剂用于纯化寡聚酶：固相支持物上杂交的实用性。
Immobilized subunits can function as an affinity sorbent to purify oligomeric enzymes: the usefulness of hybridization on the solid support.
作者信息
Muronetz V I, Ashmarina L I, Nagradova N K
出版信息
Experientia. 1981 Jan 15;37(1):15-6. doi: 10.1007/BF01965542.
PMID:7009196
Abstract
Immobilized dimers of yeast glyceraldehyde-3-phosphate dehydrogenase covalently bound to sepharose were shown to form hybrids with soluble dimers of the homologous enzymes present in crude tissue extracts (rats skeletal muscle, rat, rabbit and bovine hearts, rat liver, rat brain). Immobilized hybrid tetramers were then dissociated to form purified soluble enzymes.
摘要
已证明，与琼脂糖共价结合的酵母甘油醛-3-磷酸脱氢酶固定化二聚体可与粗组织提取物（大鼠骨骼肌、大鼠、兔和牛心脏、大鼠肝脏、大鼠脑）中存在的同源酶的可溶性二聚体形成杂交体。然后将固定化杂交四聚体解离以形成纯化的可溶性酶。
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