Fujisawa T, Waldman S R, Yonemoto R H
Gan. 1980 Apr;71(2):165-72.
A total of 81 individuals, including 41 breast cancer patients, 20 patients with other types of malignant tumors, and 20 normal volunteers, were studied in order to evaluate whether leucocyte reactivity directed to soluble tumor-associated antigens could be increased by extensive cell washings and could be blocked by preincubation with autologous serum. When 41 leucocyte samples were pretreated with autologous serum, washed 3 or 6 times, mean percentage of leucocyte adherence inhibition difference between control extract and breast cancer extract were -2.4 +/- 2.0, 5.8 +/- 1.3, and 10.0 +/- 1.2, respectively. The results of cells preincubated with serum were significantly different from those of cells washed 3 times and 6 times (both P less than 0.001 by Student's t-test). The results of cells washed 3 times were significantly different from those of cells washed 6 times (P less than 0.05 by Student's t-test). Serum blocking factors correlated with tumor burden and clinical stage. However, leucocyte reactivity against breast cancer extract did not correlate with tumor burden or clinical stage in breast cancer patients.
共有81人参与研究，其中包括41名乳腺癌患者、20名其他类型恶性肿瘤患者以及20名正常志愿者，目的是评估针对可溶性肿瘤相关抗原的白细胞反应性是否可通过大量细胞洗涤而增强，以及是否可被自体血清预孵育所阻断。当41份白细胞样本用自体血清预处理、洗涤3次或6次后，对照提取物与乳腺癌提取物之间白细胞黏附抑制差异的平均百分比分别为-2.4±2.0、5.8±1.3和10.0±1.2。血清预孵育细胞的结果与洗涤3次和6次的细胞结果有显著差异（经学生t检验，P均小于0.001）。洗涤3次的细胞结果与洗涤6次的细胞结果有显著差异（经学生t检验，P小于0.05）。血清阻断因子与肿瘤负荷和临床分期相关。然而，乳腺癌患者中白细胞对乳腺癌提取物的反应性与肿瘤负荷或临床分期无关。
