利用热解-气-液色谱-质谱联用技术对肺炎克雷伯菌菌株进行鉴别与表征
Differentiation and characterization of Klebsiella pneumoniae strains by pyrolysis-gas-liquid chromatography-mass spectrometry.
作者信息
Abbey L E, Highsmith A K, Moran T F, Reiner E J
出版信息
J Clin Microbiol. 1981 Feb;13(2):313-9. doi: 10.1128/jcm.13.2.313-319.1981.
Abstract
Nine coded duplicate strains of capsular nontypable Klebsiella pneumoniae were analyzed by pyrolysis-gas-liquid chromatography-mass spectrometry. All duplicate strains were correctly matched, and individual strains, including seven nontypable strains, were clearly distinguishable from one another. The addition of mass spectrometry to the analysis has aided the process of identification and has provided chemical structural information on K. pneumoniae strains. Application of this technique to the identification of some disease outbreaks or nosocomial problems could be of epidemiological importance, especially when conventional methods do not identify the epidemic strain.
摘要
采用热解-气-液色谱-质谱联用技术对9株编码的荚膜无定型肺炎克雷伯菌重复菌株进行了分析。所有重复菌株均正确匹配，包括7株无定型菌株在内的各个菌株之间能够清晰区分。在分析中加入质谱技术有助于鉴定过程，并提供了肺炎克雷伯菌菌株的化学结构信息。将该技术应用于某些疾病暴发或医院感染问题的鉴定可能具有流行病学意义，尤其是在传统方法无法鉴定流行菌株时。
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