Jackson R M, Heginbothom M L, Magee J T
Department of Medical Microbiology, University Hospital of Wales, Cardiff, UK.
Zentralbl Bakteriol. 1997 Jan;285(2):252-7. doi: 10.1016/s0934-8840(97)80032-1.
Thirteen isolates of ceftazidime-resistant Klebsiella pneumoniae from a suspected cross-infection outbreak involving patients on an intensive care unit and a haematology ward were examined in pyrolysis-mass spectrometry (Py-MS), along with eight concurrent non-outbreak-associated clinical isolates of klebsiellae as controls. Py-MS showed tight clustering of the suspected outbreak isolates, suggesting cross-infection with a single strain. Non-outbreak isolates were clearly distinct from one another and from the outbreak strain. The results confirm that Py-MS is a powerful tool for rapid strain comparison in investigations of cross-infection incidents.
对从涉及重症监护病房和血液科病房患者的疑似交叉感染暴发中分离出的13株耐头孢他啶肺炎克雷伯菌进行了热解质谱分析（Py-MS），同时将8株同期与暴发无关的克雷伯菌临床分离株作为对照。Py-MS显示，疑似暴发分离株紧密聚类，提示为单一菌株的交叉感染。非暴发分离株彼此之间以及与暴发菌株明显不同。结果证实，Py-MS是交叉感染事件调查中快速进行菌株比较的有力工具。
