用光亲和标记法在酿酒酵母的耐溴化乙锭菌株中诱导小菌落“突变体”。区分早期和晚期步骤。

Induction of petite "mutants" in an ethidium-resistant strain of Saccharomyces cerevisiae by photoaffinity labeling. Distinction between early and late steps.

作者信息

Fukunaga M, Yielding K L

出版信息

Mutat Res. 1981 Jan;80(1):91-7. doi: 10.1016/0027-5107(81)90179-2.

Abstract

A strain of Saccharomyces cerevisiae (MH41-7B/011) was resistant to petite induction by ethidium bromide at 30 degrees, but was sensitive to induction by photolabeling with ethidium monoazide. These results suggested a defect in the mutant in metabolic activation of ethidium to account for its resistance. Synchronized cultures of both the mutant and the normal parent strains showed a substantial reduction in petite response to photolabeling in stationary phase cells which could not be accounted for by changes in cell penetration of the drug. The use of photolabeling with normal and mutant cells suggested that petite induction can be divided into early and late steps.

摘要

一株酿酒酵母（MH41-7B/011）在30摄氏度下对溴化乙锭诱导的小菌落形成具有抗性，但对单叠氮溴化乙锭光标记诱导敏感。这些结果表明，该突变体在溴化乙锭的代谢激活方面存在缺陷，以解释其抗性。突变体和正常亲本菌株的同步培养物显示，稳定期细胞对光标记的小菌落反应大幅降低，这不能用药物细胞穿透率的变化来解释。对正常细胞和突变体细胞使用光标记表明，小菌落诱导可分为早期和晚期步骤。