Fukunaga M, Yielding L W, Firth W J, Yielding K L
Mutat Res. 1980 Jun;78(2):151-7. doi: 10.1016/0165-1218(80)90094-4.
The importance of specific substituents, especially amino azide groups, for ethidium induction of petites was evaluated in resting and dividing cells of Saccharomyces cerevisiae through the study of a series of ethidium analogs. The structural requirements in resting and growing cells were found to be different, suggesting that at least two mechanisms are responsible for induction. The significance of particular substituents in the induction processes were recognized by: (1) a dependence upon the ethyl substituent at the ring nitrogen in both actively growing and in resting cells; and (2) the implication that amino substituents are important for the effect in dividing cells and especially in resting cells. Photolytic enhancement of petite induction (via a nitrene which forms a covalent linkage to a biological site) was observed for 3 of the azide analogs, which emphasizes the likelihood that metabolic activation of ethidium to a covalent complex is responsible for its effectiveness. Furthermore, these studies indicate that these monoazide analogs should be ideal probes for examining the mitochondrial mutagenic processes.
通过对一系列溴化乙锭类似物的研究，在酿酒酵母的静止细胞和分裂细胞中评估了特定取代基，尤其是氨基叠氮基团对溴化乙锭诱导小菌落形成的重要性。发现静止细胞和生长细胞中的结构要求不同，这表明至少有两种机制负责诱导。特定取代基在诱导过程中的重要性体现在：（1）在活跃生长细胞和静止细胞中，均依赖于环氮上的乙基取代基；（2）这意味着氨基取代基对分裂细胞尤其是静止细胞中的效应很重要。在3种叠氮类似物中观察到了小菌落诱导的光解增强（通过与生物位点形成共价键的氮烯），这强调了溴化乙锭代谢活化为共价复合物是其有效性的原因的可能性。此外，这些研究表明，这些单叠氮类似物应该是用于研究线粒体诱变过程的理想探针。
