Demonstration of canine distemper viral antigen in paraffin sections, using an unlabeled antibody-enzyme method.

A peroxidase technique was used for the immunohistochemic demonstration of canine distemper viral antigen in formalin-fixed and paraffin-embedded tissues. The antigen was detected in epithelial cells and nerve cells of young dogs. The application of a combined peroxidase and hematoxylin and eosin stain as a diagnostic technique is discussed.