MaCartney L, MaCartney C M
Res Vet Sci. 1986 Mar;40(2):201-8. doi: 10.1016/S0034-5288(18)30513-7.
Two methods of immunocytochemistry, immunofluorescence (IFA) and immunoperoxidase (PAP) were used to demonstrate canine parvovirus (CPV) antigens in sections of canine tissue. Specific staining using IFA and PAP was successful only in sections of fresh frozen tissue and formalin fixed/formol sublimate postfixed tissues respectively. A range of tissues was then taken at post mortem examination from a puppy which had been experimentally infected with CPV. Upon comparison, PAP staining gave high resolution, a permanent preparation and clear intracellular localisation of antigen. IFA resulted in less defined localisation of antigen but the technique was simpler and more easily controlled.
采用免疫荧光法（IFA）和免疫过氧化物酶法（PAP）这两种免疫细胞化学方法，在犬组织切片中检测犬细小病毒（CPV）抗原。结果显示，IFA和PAP特异性染色分别仅在新鲜冷冻组织切片和福尔马林固定/升汞后固定组织切片中成功。随后，对一只经实验感染CPV的幼犬进行尸检，采集了一系列组织。经比较，PAP染色具有高分辨率、可永久保存以及抗原细胞内定位清晰的特点。IFA检测抗原的定位不太明确，但该技术更简单且易于控制。
