Martin W, Ziegler C
Blut. 1981 Jan;42(1):23-6. doi: 10.1007/BF01080260.
A method for the determination of Bf phenotypes of means of immunofixation after electrophoresis on cellogel membranes is reported. In comparison to immunofixation after agarose gel thin layer electrophoresis, this method shows an equal separation quality, but requires only about 1/5 of the monospecific anti-Bf serum, and results can be obtained after only 5 h. The gene frequencies calculated from our material (BfS 0.7994, BfF 0.1850, BfSO,7 0.0085, BfF1 0.0071) are in good accordance with investigations about other European populations.
本文报道了一种通过在细胞凝胶膜上进行电泳后免疫固定法来测定Bf表型的方法。与琼脂糖凝胶薄层电泳后的免疫固定法相比,该方法具有相同的分离质量,但仅需约五分之一的单特异性抗Bf血清,且仅5小时后即可获得结果。根据我们的材料计算出的基因频率(BfS 0.7994,BfF 0.1850,BfS0,7 0.0085,BfF1 0.0071)与其他欧洲人群的研究结果高度一致。