Blakemore W F, Murray J A
J Neurol Sci. 1981 Feb;49(2):273-84. doi: 10.1016/0022-510x(81)90084-8.
The internodal length of remyelinated internodes was examined by observations on teased CNS nerve fibres following primary demyelination induced by intraspinal injections of lysolecithin into the white matter of cats. A remyelinated internode was identified as a thinly-myelinated internode, where a node of Ranvier was bounded by a thickly-myelinated internode on one side and a less-thickly-myelinated internode on the other; as an internode of less than 100 microns in length or below 2 standard deviations from the normal regression line of internodal length against fibre diameter; as an internode joined to one of similar myelin sheath thickness which fulfilled either or both of the previous two criteria. Using the above criteria, remyelinated internodes were found to be shorter than normal; some were very short with no relationship to axon diameter while others were longer, falling within the predicted range for a given fibre diameter. This study illustrates that examination of teased CNS fibres in pathological situations can yield valuable information. However, sampling and technical difficulties are far greater than in comparable studies on peripheral nerve fibres.
通过对猫白质内注射溶血卵磷脂诱导原发性脱髓鞘后分离的中枢神经系统神经纤维进行观察，检测了再髓鞘化节间的节间长度。再髓鞘化节间被定义为薄髓鞘节间，即郎飞结一侧由厚髓鞘节间界定，另一侧由较薄髓鞘节间界定；节间长度小于100微米或低于节间长度与纤维直径正常回归线的2个标准差；节间与髓鞘厚度相似且符合前两个标准之一或两者的节间相连。使用上述标准，发现再髓鞘化节间比正常节间短；一些非常短，与轴突直径无关，而另一些较长，落在给定纤维直径的预测范围内。这项研究表明，在病理情况下检查分离的中枢神经系统纤维可以获得有价值的信息。然而，采样和技术困难远大于对外周神经纤维的类似研究。
