Sokolova I G, Malashenko Iu R, Romanovskaia V A
Mikrobiologiia. 1981 Jan-Feb;50(1):13-20.
The electron transport chain was studied in the obligate methane oxidizing culture of Methylococcus thermophilus during the oxidation of methanol (the source of carbon) which is an oxidized derivative of methane as well as during the oxidation of hydroxylamine which is an intermediate in the oxidation of ammonium (the source of nitrogen) by Mc. thermophilus cells. Cytochromes a, b and c are involved in electron transport. Cytochrome cco and cytochrome c554 have been isolated from the cell-free extract of Mc. thermophilus and purified. A scheme for electron transport operating in the oxidation of methanol and hydroxylamine is suggested on the basis of studying the characteristics of these cytochromes. Cytochrome a was shown to be a component of terminal oxidase. Cytochromes b are connected with membranes and also found in the composition of hydroxylamine oxidase. Cytochrome cco and, possibly, terminal oxidase (cytochromes a) are involved, in the oxidation of CH3OH by methanol dehydrogenase, in electron transport; cytochrome c554 as well as cytochrome b and c in the composition of hydroxylamine oxidase participate in electron transport in the oxidation of NH2OH by hydroxylamine oxidase. The characteristics of the electron transport system in Mc. thermophilus are discussed.
在嗜热甲基球菌专性甲烷氧化培养物中，研究了甲醇（碳源，甲烷的氧化衍生物）氧化过程以及羟胺氧化过程中的电子传递链。羟胺是嗜热甲基球菌细胞氧化铵（氮源）过程中的一种中间产物。细胞色素a、b和c参与电子传递。细胞色素cco和细胞色素c554已从嗜热甲基球菌的无细胞提取物中分离并纯化。在研究这些细胞色素特性的基础上，提出了甲醇和羟胺氧化过程中电子传递的运作模式。细胞色素a被证明是末端氧化酶的一个组成部分。细胞色素b与膜相连，也存在于羟胺氧化酶的组成中。细胞色素cco以及可能的末端氧化酶（细胞色素a）参与甲醇脱氢酶将CH3OH氧化过程中的电子传递；细胞色素c554以及羟胺氧化酶组成中的细胞色素b和c参与羟胺氧化酶将NH2OH氧化过程中的电子传递。文中讨论了嗜热甲基球菌中电子传递系统的特性。
