Ishiguro A, Weeks G
J Biol Chem. 1978 Nov 10;253(21):7585-7.
Discoidin I, the developmentally regulated carbohydrate binding protein from Dictyostelium discoideum, migrated as a broad band during electrophoretic analysis using 10% polyacrylamide gels in the presence of sodium dodecyl sulfate. The band could be resolved into two components by electrophoresis on 15% polyacrylamide gels. All highly purified preparations of discoidin I contained equal amounts of the two components, suggesting they are nonidentical subunits of the protein. The subunit molecular weights of discoidin I and discoidin II were found to be 29,500 and 26,500, values somewhat larger than those previously reported.
盘基网柄菌发育调控的碳水化合物结合蛋白盘基网柄菌凝集素I，在十二烷基硫酸钠存在的情况下，使用10%聚丙烯酰胺凝胶进行电泳分析时呈现为一条宽带。通过在15%聚丙烯酰胺凝胶上进行电泳，这条带可被解析为两个组分。所有高度纯化的盘基网柄菌凝集素I制剂都含有等量的这两个组分，这表明它们是该蛋白质的不同亚基。盘基网柄菌凝集素I和盘基网柄菌凝集素II的亚基分子量分别为29,500和26,500，这些值比先前报道的略大。
