Andersen P, Langmoen I A
Q Rev Biophys. 1980 Feb;13(1):1-18. doi: 10.1017/s0033583500000299.
Brain slice preparations seem to offer an opportunity to study the mechanism of action of some drugs. They allow long-lasting intracellular recording of good quality. Measurement of membrane parameters may be made under conditions in which drugs may be applied to the bath, by the microdrop method or by one or several iontophoretic electrode assembles to various positions on the same cell. Combined with microsurgery and various pre-treatments of the animal, the slice technique may extent our knowledge on the detailed mechanisms of drug interaction with particular neurons. A useful development of this technique probably requires a continuous comparison between the results obtained from slices with those acquired from experiments on the same cell types in intact preparations.
脑片制备似乎为研究某些药物的作用机制提供了一个机会。它们能够进行长时间高质量的细胞内记录。膜参数的测量可以在将药物应用于浴槽的条件下进行，通过微量滴注法或通过一个或几个离子电泳电极组件施加到同一细胞的不同位置。结合显微手术和对动物的各种预处理，切片技术可以扩展我们对药物与特定神经元相互作用详细机制的认识。这项技术的一个有益发展可能需要持续比较从切片获得的结果与从完整制剂中相同细胞类型的实验获得的结果。
